CA9 Protein (AA 38-402) (His tag)

N° du produit ABIN7274013

Détail du produit

Antigène: CA9 (Carbonic Anhydrase IX (CA9))

Type de proteíne: Recombinant

Attributs du protein: AA 38-402

Origine: Cynomolgus

Source: HEK-293 Cells

Purification/Conjugué: Cette CA9 protéine est marqué à la His tag.

Fonction: Cynomolgus CA9/Carbonic Anhydrase IX Protein

Séquence: Gln38-Asp402

Attributs du produit: Recombinant Cynomolgus CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln38-Asp402.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus CA9, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CA9 (Carbonic Anhydrase IX (CA9))

Autre désignation: CA9 (CA9 Produits)

Synonymes: CA9 Protein, CAIX Protein, Ca9 Protein, MN/CA9 Protein, MN Protein, carbonic anhydrase 9 Protein, CA9 Protein, CAH9 Protein, Car9 Protein

Sujet: CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.

Poids moléculaire: 41.03 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5VQG9