CD83 Protein (CD83) (AA 20-143) (His tag)

N° du produit ABIN7274248

Détail du produit

Antigène: CD83

Type de proteíne: Recombinant

Attributs du protein: AA 20-143

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD83 protéine est marqué à la His tag.

Fonction: Human CD83 Protein

Séquence: Thr20-Ala143

Attributs du produit: Recombinant Human CD83 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Ala143.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD83, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD83

Autre désignation: CD83 (CD83 Produits)

Synonymes: BL11 Protein, HB15 Protein, CD83 molecule Protein, CD83 antigen Protein, Cd83 Protein, CD83 Protein

Sujet: CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear.

Poids moléculaire: 15.2 kDa. Due to glycosylation, the protein migrates to 18 kDa and 25-28 kDa based on Tris-Bis PAGE result.