CD28 Protein (CD28) (AA 19-152) (His-Avi Tag,Biotin)

N° du produit ABIN7274256

Cette protéine Recombinant CD28 est produite dans HEK-293 Cells.

Aperçu rapide pour CD28 Protein (CD28) (AA 19-152) (His-Avi Tag,Biotin) (ABIN7274256)

Antigène: CD28

Type de proteíne: Recombinant

Origine: Humain, Cynomolgus, Macaque rhésus

Source: HEK-293 Cells

Pureté: > 95 % as determined by Tris-Bis PAGE

Détail du produit

Attributs du protein: AA 19-152

Purification/Conjugué: Cette CD28 protéine est marqué à la His-Avi Tag,Biotin.

Fonction: Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein

Séquence: Asn19-Pro152

Attributs du produit: Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-CD28 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24.4ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD28

Autre désignation: CD28

Sujet: CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Poids moléculaire: 18.2 kDa. Due to glycosylation, the protein migrates to 38-55 kDa based on Tris-Bis PAGE result.

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response