CD28 Protein (CD28) (AA 19-152) (mFc Tag)

N° du produit ABIN7274254

Détail du produit

Antigène: CD28

Type de proteíne: Recombinant

Attributs du protein: AA 19-152

Origine: Humain, Cynomolgus, Macaque rhésus

Source: HEK-293 Cells

Purification/Conjugué: Cette CD28 protéine est marqué à la mFc Tag.

Fonction: Human/Cynomolgus/Rhesus macaque CD28 Protein

Séquence: Asn19-Pro152

Attributs du produit: Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-Terminus.It contains Asn19-Pro152.

Pureté: > 95 % as determined by Tris-Bis PAGE

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human/Cynomolgus/Rhesus macaque CD28, mFc (IgG2a) Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 23.3ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD28

Autre désignation: CD28 (CD28 Produits)

Synonymes: Tp44 Protein, CD28RNA Protein, cd28 Protein, CD28 molecule Protein, CD28 antigen Protein, Cd28 molecule Protein, CD28 molecule L homeolog Protein, CD28 Protein, Cd28 Protein, cd28.L Protein

Sujet: CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Poids moléculaire: 41.9 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response