CHRDL1 Protein (AA 28-456) (His tag)

N° du produit ABIN7274317

Détail du produit

Antigène: CHRDL1 (Chordin-Like 1 (CHRDL1))

Type de proteíne: Recombinant

Attributs du protein: AA 28-456

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette CHRDL1 protéine est marqué à la His tag.

Fonction: Human CHRDL1 Protein

Séquence: Glu28-Cys456

Attributs du produit: Recombinant Human CHRDL1 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Glu28-Cys456.

Pureté: > 90 % as determined by Tris-Bis PAGE

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CHRDL1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CHRDL1 Antibody, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 4 mM HCl.

Buffer: Lyophilized from 0.22μm filtered solution in 4 mM HCl. Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CHRDL1 (Chordin-Like 1 (CHRDL1))

Autre désignation: CHRDL1 (CHRDL1 Produits)

Synonymes: CHL Protein, NRLN1 Protein, VOPT Protein, dA141H5.1 Protein, CHL1 Protein, Nrln1 Protein, chl Protein, vopt Protein, nrln1 Protein, da141h5.1 Protein, Kjr Protein, Neurogenesin-1 Protein, Ng1 Protein, chordin like 1 Protein, chordin-like 1 Protein, CHRDL1 Protein, Chrdl1 Protein, chrdl1 Protein

Sujet: CHRDL1 (Chordin-like 1) is a secreted protein that acts as an antagonist of bone morphogenetic protein (BMP). BMP plays a role as an activator of BMP receptor II (BMPR II), which mediates extracellular to intracellular signal transmission and is involved in carcinogenesis and metastasis.The hypermethylation of the CHRDL1 promoter in gastric cancer, which induced low expression of CHRDL1 and decreased its secretion to the supernatant.

Poids moléculaire: 50 kDa same as Tris-Bis PAGE result.