CD226 Protein (CD226) (AA 19-247) (Fc Tag)

N° du produit ABIN7274457

Détail du produit

Antigène: CD226

Type de proteíne: Recombinant

Attributs du protein: AA 19-247

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD226 protéine est marqué à la Fc Tag.

Fonction: Human DNAM-1/CD226 Protein

Séquence: Glu19-Asn247

Attributs du produit: Recombinant Human DNAM-1/CD226 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu19-Asn247.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human CD155, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 4.8μg/ml determined by ELISA. The affinity constant of 0.234 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD226

Autre désignation: DNAM-1 (CD226 Produits)

Synonymes: CD226 Protein, cd226 Protein, zgc:158817 Protein, BC051526 Protein, DNAM-1 Protein, DNAM1 Protein, Pta1 Protein, TLiSA1 Protein, PTA1 Protein, CD226 molecule Protein, CD226 antigen Protein, CD226 Protein, cd226 Protein, Cd226 Protein

Sujet: DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

Poids moléculaire: 52.8 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result.

UniProt: Q15762

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints