IL-6 Protein (AA 30-212)

N° du produit ABIN7274962

Cette protéine Recombinant IL-6 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL-6 Protein (AA 30-212) (ABIN7274962)

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Détail du produit

Attributs du protein: AA 30-212

Fonction: Human IL-6 Protein

Séquence: Val30-Met212

Attributs du produit: Recombinant Human IL-6 is expressed from E.coli without tag.It contains Val30-Met212.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 0.05 EU per μg by the LAL method.

Biological Activity Comment: Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-6 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA.,Human IL-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1 - 0.8 ng/mL.,Loaded Human IL-6 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-6, No Tag with an affinity constant of 16.50 nM as determined in BLI assay (Gator® Prime).,The purity of Human IL-6 is greater than 90% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Autre désignation: IL-6

Sujet: IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.

Poids moléculaire: The protein has a predicted 20.8 kDa same as Bis-Tris PAGE result.

UniProt: P05231

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome