IL-6 Protein (AA 30-212)

N° du produit ABIN7274962

Cette protéine Recombinant IL-6 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL-6 Protein (AA 30-212) (ABIN7274962)

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC

Détail du produit

Attributs du protein: AA 30-212

Fonction: Human IL-6 Protein

Séquence: Val30-Met212

Attributs du produit: Recombinant Human IL-6 is expressed from E.coli without tag.It contains Val30-Met212.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 0.05 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-6 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 32.6ng/ml determined by ELISA. The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Autre désignation: IL-6

Sujet: IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.

Poids moléculaire: The protein has a predicted 20.8 kDa same as Tris-Bis PAGE result.

UniProt: P05231

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome