Cette IL-17A/F protéine est marqué à la His-Avi Tag,Biotin.
Séquence
Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)
Pureté
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Biotinylated Human IL-17A&F, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 0.19μg/ml determined by ELISA. See testing image for detail.
IL-17A/F
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
Purity is >98 % , as determined by Coomassie stained SDS-PAGE.
CyTOF, FACS
Active
IL-17A/F
Origine: Humain
Hôte: Human Cells
Recombinant
Greater than 95 % as determined by reducing SDS-PAGE.
ELISA, MS, SDS, WB
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Stock
4 °C,-80 °C
Stockage commentaire
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
il17-5 Protein, si:ch211-63o20.2 Protein, interleukin 17a/f3 Protein, il17a/f3 Protein
Sujet
IL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,IL24,ML-1,Interleukin-17F,Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Poids moléculaire
15.1 kDa (IL-17A)&17.5 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa&27 kDa based on Tris-Bis PAGE result.