CD51/CD61 Protein (AA 31-992) (His-Avi Tag,Biotin)

N° du produit ABIN7275060

Détail du produit

Antigène: CD51/CD61 (ITGAV/ITGB3) (Integrin AlphaV, Integrin Beta3 (ITGAV/ITGB3))

Type de proteíne: Recombinant

Attributs du protein: AA 31-992

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD51/CD61 protéine est marqué à la His-Avi Tag,Biotin.

Fonction: Biotinylated Human Integrin alpha V beta 3 (ITGAV & ITGB3) Heterodimer Protein

Séquence: Phe31-Val992 (ITGAV) acidic tail & Gly27-Asp718 (ITGB3) basic tail

Specificité: Uni-Prot: P06756-1 (ITGAV), P05106-1 (ITGB3)

Attributs du produit: Recombinant Biotinylated Human Integrin alpha V beta 3 (ITGAV & ITGB3) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe31-Val992 (ITGAV) acidic tail & Gly27-Asp718 (ITGB3) basic tail.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-alpha V beta3 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB3, His Tag with the EC50 of 54ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD51/CD61 (ITGAV/ITGB3) (Integrin AlphaV, Integrin Beta3 (ITGAV/ITGB3))

Autre désignation: ITGAV&ITGB3 (ITGAV/ITGB3 Produits)

Synonymes: CD51 Protein, MSK8 Protein, VNRA Protein, VTNR Protein, integrin subunit alpha V Protein, ITGAV Protein

Sujet: ITGAV&ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV&ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV&ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV&ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV&ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling.

Poids moléculaire: 114 kDa (ITGAV) & 81.3 kDa (ITGB3). Due to glycosylation, the protein migrates to 100-160 kDa based on Tris-Bis PAGE result.