MADCAM1 Protein (AA 19-317) (His tag)

N° du produit ABIN7275237

Détail du produit

Antigène: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Type de proteíne: Recombinant

Attributs du protein: AA 19-317

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette MADCAM1 protéine est marqué à la His tag.

Fonction: Human MADCAM1 Protein

Séquence: Gln19-Gln317

Attributs du produit: Recombinant Human MADCAM1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Gln317.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human MADCAM1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Autre désignation: MADCAM1 (MADCAM1 Produits)

Synonymes: MACAM1 Protein, AV211525 Protein, mucosal vascular addressin cell adhesion molecule 1 Protein, MADCAM1 Protein, Madcam1 Protein

Sujet: Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) contributes to the recruitment of donor T cells into the mucosal tissues of the recipient after allogeneic hematopoietic stem cell transplantation (aHSCT). The aim of our study was to determine whether selected single nucleotide polymorphisms (SNPs) of the MADCAM1 gene are associated with development of serious complications after aHSCT.

Poids moléculaire: 32.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.