ROBO4 Protein (AA 27-469) (His tag)

N° du produit ABIN7275523

Détail du produit

Antigène: ROBO4 (Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4))

Type de proteíne: Recombinant

Attributs du protein: AA 27-469

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette ROBO4 protéine est marqué à la His tag.

Fonction: Human ROBO4 Protein

Séquence: Ala27-Glu469

Attributs du produit: Recombinant Human ROBO4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala27-Glu469.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human ROBO4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 26.1ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: ROBO4 (Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4))

Autre désignation: ROBO4 (ROBO4 Produits)

Synonymes: ECSM4 Protein, MRB Protein, 1200012D01Rik Protein, AI593217 Protein, sc:d0823 Protein, wu:fa16f09 Protein, roundabout guidance receptor 4 Protein, roundabout, axon guidance receptor, homolog 4 (Drosophila) Protein, ROBO4 Protein, Robo4 Protein, robo4 Protein

Sujet: Roundabout4 (Robo4) is a transmembrane receptor that belongs to the Roundabout (Robo) family of axon guidance molecules. Robo4 is an endothelial-specific receptor that participates in endothelial cell migration, proliferation, and angiogenesis and the maintenance of vasculature homeostasis. Robo4 is a promising and potentially valuable therapeutic target for treating pathological angiogenesis and developmental defects in angiogenesis.

Poids moléculaire: 47.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.