CD33 Protein (CD33) (AA 18-259) (Fc Tag)

N° du produit ABIN7275588

Détail du produit

Antigène: CD33

Type de proteíne: Recombinant

Attributs du protein: AA 18-259

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD33 protéine est marqué à la Fc Tag.

Fonction: Human Siglec-3/CD33 Protein

Séquence: Asp18-His259

Attributs du produit: Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp18-His259.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Siglec-3 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 33.9ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD33

Autre désignation: Siglec-3 (CD33 Produits)

Synonymes: SIGLEC-3 Protein, SIGLEC3 Protein, p67 Protein, Siglec-3 Protein, gp67 Protein, CD33 molecule Protein, CD33 antigen Protein, CD33 Protein, Cd33 Protein

Sujet: Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.

Poids moléculaire: 53.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.