IL-3 Protein (AA 20-152)

N° du produit ABIN7454153

Cette protéine Recombinant IL-3 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL-3 Protein (AA 20-152) (ABIN7454153)

Antigène: IL-3 (Interleukin 3 (IL-3))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 20-152

Fonction: Human IL-3 Protein

Séquence: Ala20-Phe152

Attributs du produit: Recombinant Human IL-3 Protein is expressed from E.coli without tag.It contains Ala20-Phe152.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 0.05 EU per μg by the LAL method.

Biological Activity Comment: Human IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50-for this effect is 0.15 - 0.75 ng/mL (QC Test).,The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-3 (Interleukin 3 (IL-3))

Autre désignation: IL-3

Sujet: Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Poids moléculaire: 15.09 kDa same as Bis-Tris PAGE result.

Pathways: Signalistation JAK/STAT, Regulation of Carbohydrate Metabolic Process, Autophagy