MICB Protein (AA 23-298) (Fc Tag)

N° du produit ABIN7454188

Détail du produit

Antigène: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Type de proteíne: Recombinant

Attributs du protein: AA 23-298

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette MICB protéine est marqué à la Fc Tag.

Fonction: Human MICB Protein

Séquence: Ala23-Gly298

Attributs du produit: Recombinant Human MICB Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala23-Gly298.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human MICB, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-MICB Antibody, hFc Tag with the EC50 of 54.9ng/ml determined by ELISA (QC Test). The affinity constant of 95.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Autre désignation: MICB (MICB Produits)

Synonymes: PERB11.2 Protein, MHC class I polypeptide-related sequence B Protein, MHC class I-related protein-like Protein, MICB Protein, LOC512825 Protein

Sujet: MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85 % amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.

Poids moléculaire: 58.21 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.