FGF2 Protein (AA 135-288)

N° du produit ABIN7454253

Protéine Recombinant FGF2 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour FGF2 Protein (AA 135-288) (ABIN7454253)

Antigène: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 135-288

Fonction: Human FGF basic (154aa) Protein

Séquence: Ala135-Ser288

Attributs du produit: Recombinant Human FGF basic (154aa) Protein is expressed from E.coli without tag.It contains Ala135-Ser288.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 0.1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FGF basic (154aa) at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Autre désignation: FGF basic

Sujet: FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60 % amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.

Poids moléculaire: 17.12 kDa same as Tris-Bis PAGE result.

UniProt: P09038-4

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins