CD13 Protein (His tag)

N° du produit ABIN7519664

Détail du produit

Antigène: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD13 protéine est marqué à la His tag.

Fonction: Active Recombinant Human Aminopeptidase N/CD13 Protein

Séquence: KAWNRYRLPN TLKPDSYRVT LRPYLTPNDR GLYVFKGSST VRFTCKEATD VIIIHSKKLN YTLSQGHRVV LRGVGGSQPP DIDKTELVEP TEYLVVHLKG SLVKDSQYEM DSEFEGELAD DLAGFYRSEY MEGNVRKVVA TTQMQAADAR KSFPCFDEPA MKAEFNITLI HPKDLTALSN MLPKGPSTPL PEDPNWNVTE FHTTPKMSTY LLAFIVSEFD YVEKQASNGV LIRIWARPSA IAAGHGDYAL NVTGPILNFF AGHYDTPYPL PKSDQIGLPD FNAGAMENWG LVTYRENSLL FDPLSSSSSN KERVVTVIAH ELAHQWFGNL VTIEWWNDLW LNEGFASYVE YLGADYAEPT WNLKDLMVLN DVYRVMAVDA LASSHPLSTP ASEINTPAQI SELFDAISYS KGASVLRMLS SFLSEDVFKQ GLASYLHTFA YQNTIYLNLW DHLQEAVNNR SIQLPTTVRD IMNRWTLQMG FPVITVDTST GTLSQEHFLL DPDSNVTRPS EFNYVWIVPI TSIRDGRQQQ DYWLIDVRAQ NDLFSTSGNE WVLLNLNVTG YYRVNYDEEN WRKIQTQLQR DHSAIPVINR AQIINDAFNL ASAHKVPVTL ALNNTLFLIE ERQYMPWEAA LSSLSYFKLM FDRSEVYGPM KNYLKKQVTP LFIHFRNNTN NWREIPENLM DQYSEVNAIS TACSNGVPEC EEMVSGLFKQ WMENPNNNPI HPNLRSTVYC NAIAQGGEEE WDFAWEQFRN ATLVNEADKL RAALACSKEL WILNRYLSYT LNPDLIRKQD ATSTIISITN NVIGQGLVWD FVQSNWKKLF NDYGGGSFSF SNLIQAVTRR FSTEYELQQL EQFKKDNEET GFGSGTRALE QALEKTKANI KWVKENKEVV LQWFTENSK

Specificité: Lys69-Lys967

Pureté: > 98 % by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >4300 pmol/min/μg.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Stock: -20 °C,-80 °C

Stockage commentaire: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Détail du antigène

Antigène: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Autre désignation: Aminopeptidase N/CD13 (ANPEP Produits)

Synonymes: APN Protein, CD13 Protein, GP150 Protein, LAP1 Protein, P150 Protein, PEPN Protein, A2AP Protein, AAP Protein, ALPHA-2-PI Protein, API Protein, PLI Protein, AAT1 Protein, ALT1 Protein, GPT1 Protein, Gpt1 Protein, Apn Protein, pepN Protein, SC8E4A.13 Protein, 1300007J06Rik Protein, 2310022B03Rik Protein, ALT Protein, Gpt-1 Protein, AP-M Protein, AP-N Protein, Cd13 Protein, Apm Protein, KZP Protein, Lap1 Protein, rAPN Protein, APDE Protein, p150 Protein, anpep Protein, fb64c05 Protein, wu:fb64c05 Protein, zgc:136771 Protein, alanyl aminopeptidase, membrane Protein, serpin family F member 2 Protein, glutamic--pyruvic transaminase Protein, aminopeptidase N Protein, aminopeptidase Protein, aminopeptidase n Protein, glutamic pyruvic transaminase, soluble Protein, alanyl (membrane) aminopeptidase Protein, membrane alanyl aminopeptidase Protein, alanyl (membrane) aminopeptidase b Protein, alanyl aminopeptidase, membrane L homeolog Protein, ANPEP Protein, SERPINF2 Protein, GPT Protein, Gpt Protein, Apn2 Protein, Apn1 Protein, SCO2643 Protein, pepN Protein, Shewana3_0897 Protein, Alvin_1024 Protein, Ndas_3413 Protein, Saut_1544 Protein, Fbal_1877 Protein, ML5_3424 Protein, Despr_1988 Protein, Mesop_5365 Protein, Anpep Protein, LOC101744250 Protein, anpepb Protein, anpep.L Protein

Sujet:

Description: Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma.

Name: ANPEP, APN, CD13, GP150, LAP1, P150, PEPN, aminopeptidase N,APN,CD13,GP150,LAP1,P150,PEPN, AP-M, AP-N, hAPN

ID gène: 290

UniProt: P15144

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones