CA5A Protein (His tag)

N° du produit ABIN7536455

Détail du produit

Antigène: CA5A (Carbonic Anhydrase VA (CA5A))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CA5A protéine est marqué à la His tag.

Fonction: Recombinant Human Carbonic anhydrase 5A/CA5A Protein

Séquence: AWQTSNNTLH PLWTVPVSVP GGTRQSPINI QWRDSVYDPQ LKPLRVSYEA ASCLYIWNTG YLFQVEFDDA TEASGISGGP LENHYRLKQF HFHWGAVNEG GSEHTVDGHA YPAELHLVHW NSVKYQNYKE AVVGENGLAV IGVFLKLGAH HQTLQRLVDI LPEIKHKDAR AAMRPFDPST LLPTCWDYWT YAGSLTTPPL TESVTWIIQK EPVEVAPSQL SAFRTLLFSA LGEEEKMMVN NYRPLQPLMN RKVWASFQAT NEGTRS

Specificité: Ala40-Ser305

Pureté: > 92 % by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: < 0.01EU/μg

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Concentration: 0.54 mg/mL

Buffer: Lyophilized from a 0.22 μm filtered solution of 50 mM NaAc,50 mM NaCl,0.05 % Brij-35, pH 5.0.

Stock: -20 °C,-80 °C

Stockage commentaire: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Détail du antigène

Antigène: CA5A (Carbonic Anhydrase VA (CA5A))

Autre désignation: Carbonic anhydrase 5A/CA5A (CA5A Produits)

Synonymes: CAV Protein, Ca5a Protein, Car5 Protein, Ca5 Protein, CA5 Protein, CAVA Protein, carbonic anhydrase 5a, mitochondrial Protein, carbonic anhydrase 5A Protein, Car5a Protein, Ca5a Protein, CA5A Protein

Sujet:

Description: Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase VA encoded by the CA5A gene is a mitochondrial protein (2, 3). In comparison with another mitochondrial CA (CA5B), CA5A has different tissue distribution and chromosomal location (4, 5). Expression and inhibitor studies of different CAs in the rat pancreatic beta cells indicate that CA5A may be involved in the regulation of insulin secretion (6). CA5A may also participate in the detoxification of ammonia produced in the gastrointestinal tract by providing bicarbonate to carbamyl phosphate synthetase I (7). The amino acid sequence of recombinant human CA5A (residues 40 to 305) is 79 % , 77 %, and 76 % identical to that of canine, bovine, and rat/mouse.

Name: CA5, CAV, CAVA, CA5AD, GS1-21A4.1,CA5A

ID gène: 763

UniProt: P35218