CST6 Protein (His tag)

N° du produit ABIN7534344

Détail du produit

Antigène: CST6 (Cystatin E/M (CST6))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CST6 protéine est marqué à la His tag.

Fonction: Active Recombinant Human Cystatin-M/CST6 Protein

Séquence: RPQERMVGEL RDLSPDDPQV QKAAQAAVAS YNMGSNSIYY FRDTHIIKAQ SQLVAGIKYF LTMEMGSTDC RKTRVTGDHV DLTTCPLAAG AQQEKLRCDF EVLVVPWQNS SQLLKHNCVQ M

Specificité: Arg29-Met149

Pureté: > 95 % by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: < 0.01 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is approximately 16 nM.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Stock: -20 °C,-80 °C

Stockage commentaire: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Détail du antigène

Antigène: CST6 (Cystatin E/M (CST6))

Autre désignation: Cystatin-M/CST6 (CST6 Produits)

Synonymes: CST6 Protein, 1110017E11Rik Protein, N28197 Protein, ichq Protein, cystatin E/M Protein, CST6 Protein, Cst6 Protein

Sujet:

Description: Cystatin E/M encoded by the CST6 gene is a member of family 2 of the cystatin superfamily. It inhibits papain and cathepsin B, two of the cysteine proteases. Its mRNA was found in many tissues by the two groups who did initial cloning. However, its protein was found only in skin and sweat glands by a third group. In addition to being a cysteine protease inhibitor, cystatin E/M is also a substrate for transglutaminases. It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice, with a null mutation in the cystatin E/M gene, have defects in epidermal cornification and die between 5 and 12 days of age. Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation.

Name: CST6

ID gène: 1474

UniProt: Q15828