Fibronectin 1 Protein (FN1) (His tag)

N° du produit ABIN7535490

Détail du produit

Antigène: Fibronectin 1 (FN1)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette Fibronectin 1 protéine est marqué à la His tag.

Fonction: Active Recombinant Human Fibronectin/CIG/FN1 Protein

Séquence: SSSGPVEVFI TETPSQPNSH PIQWNAPQPS HISKYILRWR PKNSVGRWKE ATIPGHLNSY TIKGLKPGVV YEGQLISIQQ YGHQEVTRFD FTTTSTSTPV TSNTVTGETT PFSPLVATSE SVTEITASSF VVSWVSASDT VSGFRVEYEL SEEGDEPQYL DLPSTATSVN IPDLLPGRKY IVNVYQISED GEQSLILSTS QTTAPDAPPD TTVDQVDDTS IVVRWSRPQA PITGYRIVYS PSVEGSSTEL NLPETANSVT LSDLQPGVQY NITIYAVEEN QESTPVVIQQ ETTGTPRSDT VPSPRDLQFV EVTDVKVTIM WTPPESAVTG YRVDVIPVNL PGEHGQRLPI SRNTFAEVTG LSPGVTYYFK VFAVSHGRES KPLTAQQTTK LDAPTNLQFV NETDSTVLVR WTPPRAQITG YRLTVGLTRR GQPRQYNVGP SVSKYPLRNL QPASEYTVSL VAIKGNQESP KATGVFTTLQ PGSSIPPYNT EVTETTIVIT WTPAPRIGFK LGVRPSQGGE APREVTSDSG SIVVSGLTPG VEYVYTIQVL RDGQERDAPI VNKVVTPLSP PTNLHLEANP DTGVLTVSWE RSTTPDITGY RITTTPTNGQ QGNSLEEVVH ADQSSCTFDN LSPGLEYNVS VYTVKDDKES VPISDTIIP

Specificité: Ser607-Pro1265

Pureté: > 90 % by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human Fibronectin at 1 μg/mL (100 μL/well) can bind Fibronectin Rabbit mAb with a linear range of 0.1-0.89 ng/mL.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Stock: -20 °C,-80 °C

Stockage commentaire: Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Détail du antigène

Antigène: Fibronectin 1 (FN1)

Autre désignation: Fibronectin/CIG/FN1 (FN1 Produits)

Synonymes: FN1 Protein, FN Protein, cig Protein, fibronectin Protein, finc Protein, lets Protein, msf Protein, Fn Protein, fn2 Protein, fb80d10 Protein, wu:fb80d10 Protein, CIG Protein, ED-B Protein, FINC Protein, FNZ Protein, GFND Protein, GFND2 Protein, LETS Protein, MSF Protein, E330027I09 Protein, Fn-1 Protein, FIBNEC Protein, fn-1 Protein, fibronectin 1 Protein, fibronectin 1a Protein, fibronectin 1 S homeolog Protein, FN1 Protein, fn1 Protein, fn1a Protein, Fn1 Protein, fn1.S Protein

Sujet:

Description: Fibronectin (FN) is a glycoprotein component of the extracellular matrix of the extracellular matrix (ECM) with roles in embryogenesis, development, and wound healing. More recently, FN has emerged as player in platelet thrombus formation and diseases associated with thrombosis including vascular remodeling, atherosclerosis, and cardiac repair following a myocardial infarct. Each monomer of FN consists of three types of homologous repeating units, that is 12 type I repeats, two type II repeats and 15-17 type III repeats. The occurrence of multiple isoforms results from alternative mRNA splicing of the ED-A, ED-B and III-CS regions, and subsequent post-translational modification. As an ECM component and one of the primary cell adhesion molecules, Fibronectin can be a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, as well as many integrin receptors through which FN mediates the variety of cellular signaling pathways. The study of solid human tumors showed among the early signs of malignant transformation the fragmentation of pericellular FN, concommitent with the increase of its production by the peritumoral stroma. These results should encourage further investigations concerning the potential importance of Fn production and breakdown during cancer progression. FN1 expression has been described to increase significantly from the morula towards the early blastocyst stage, suggesting that FN1 may also be involved in early blastocyst formation. The fragment 2 of FN comprises the first 7 FN type III repeats and is suggested to be important for self association during fibril growth via the key module III2.

Name: FN1,CIG,ED-B,FINC,FN,FNZ,GFND,GFND2,LETS,MSF,fibronectin

ID gène: 2335

UniProt: P02751

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy