LGMN Protein (His tag)

N° du produit ABIN7535954

Détail du produit

Antigène: LGMN (Legumain (LGMN))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Purification/Conjugué: Cette LGMN protéine est marqué à la His tag.

Fonction: Active Recombinant Mouse Asparaginyl endopeptidase/Legumain Protein

Séquence: VPVGVDDPED GGKHWVVIVA GSNGWYNYRH QADACHAYQI IHRNGIPDEQ IIVMMYDDIA NSEENPTPGV VINRPNGTDV YKGVLKDYTG EDVTPENFLA VLRGDAEAVK GKGSGKVLKS GPRDHVFIYF TDHGATGILV FPNDDLHVKD LNKTIRYMYE HKMYQKMVFY IEACESGSMM NHLPDDINVY ATTAANPKES SYACYYDEER GTYLGDWYSV NWMEDSDVED LTKETLHKQY HLVKSHTNTS HVMQYGNKSI STMKVMQFQG MKHRASSPIS LPPVTHLDLT PSPDVPLTIL KRKLLRTNDV KESQNLIGQI QQFLDARHVI EKSVHKIVSL LAGFGETAER HLSERTMLTA HDCYQEAVTH FRTHCFNWHS VTYEHALRYL YVLANLCEAP YPIDRIEMAM DKVCLSHY

Specificité: Val18-Tyr435

Pureté: > 95 % by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: <0.1EU/μg

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin(Z-AAN-AMC). The specific activity is > 107 pmol/min/μg.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Stock: -20 °C,-80 °C

Stockage commentaire: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Détail du antigène

Antigène: LGMN (Legumain (LGMN))

Autre désignation: Asparaginyl endopeptidase/Legumain (LGMN Produits)

Synonymes: AEP Protein, LGMN1 Protein, PRSC1 Protein, AI746452 Protein, AU022324 Protein, Prsc1 Protein, MGC64351 Protein, cb173 Protein, cb460 Protein, sb:cb173 Protein, zgc:76953 Protein, wu:fa98g05 Protein, wu:fj92g08 Protein, lgmn Protein, MGC88980 Protein, DELTA VACUOLAR PROCESSING ENZYME Protein, DELTAVPE Protein, delta vacuolar processing enzyme Protein, legumain Protein, legumain L homeolog Protein, delta vacuolar processing enzyme Protein, Legumain Protein, LGMN Protein, Lgmn Protein, lgmn.L Protein, lgmn Protein, DELTA-VPE Protein, MBOO_RS07785 Protein, Odosp_3582 Protein, LOC569153 Protein

Sujet:

Description: The Mammalian Legumain, also known as LGMN, also called asparaginyl endopeptidase (AEP), is a cysteine protease belonging to peptidase family C13 with strict specificity for hydrolysis of asparaginyl bonds. Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation.

Name: AEP, Prsc1,LGMN

ID gène: 19141

UniProt: O89017

Pathways: Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades