IL-1 beta Protein (AA 114-265)

N° du produit ABIN7596223

Cette protéine Recombinant IL-1 beta est produite dans Escherichia coli (E. coli).

Aperçu rapide pour IL-1 beta Protein (AA 114-265) (ABIN7596223)

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Chien

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Activity Assay (AcA)

Pureté: > 95% by SDS-PAGE

Détail du produit

Attributs du protein: AA 114-265

Séquence: MAAMQSVDCK LQDISHKYLV LSNSYELRAL HLNGENVNKQ VVFHMSFVHG DESNNKIPVV LGIKQKNLYL SCVMKDGKPT LQLEKVDPKV YPKRKMEKRF VFNKIEIKNT VEFESSQYPN WYISTSQVEG MPVFLGNTRG GQDITDFTME FSS

niveau d'endotoxine: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 5pg/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: IL-1 beta/IL1B

Sujet: Interleukin-1 (IL-1) has been considered a potentially important inflammatory mediator. It is composed of two distinct proteins, called as IL-1alpha and IL-1beta. IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and they are structurally related polypeptides that share approximately 20 % amino acid. Recombinant canine IL-1beta was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 17.5 kDa (153aa) confirmed by MALDI-TOF

NCBI Accession: NP_001033060

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome