HMGB1 Protein (BoxAB, C-Tailless)

N° du produit ABIN7862612

Protéine Recombinant HMGB1 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour HMGB1 Protein (BoxAB, C-Tailless) (ABIN7862612)

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Activation (Act), Cellular Assay (CA)

Pureté: > 95 % It contains no nucleic acids.

Détail du produit

Attributs du protein: BoxAB, C-Tailless

Fonction: BoxAB (Tailless-HMGB1) is a recombinant fragment of HMGB1 including both DNA-binding domains, BoxA and BoxB, lacking the acidic C-terminal tail.

Séquence: BoxAB from HMGB1 consists of 176 amino acids: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVV

Attributs du produit: BoxAB from HMGB1 includes both BoxA and BoxB domains connected as in the natural sequence but lacks the negatively charged C-terminal tail. This tailless form retains the structural integrity and receptor-binding capacity of full-length HMGB1, while showing altered functional behavior in redox-dependent signaling and immune activation.
BoxAB is ideal for studying the mechanisms of HMGB1-mediated inflammation, receptor binding (RAGE, TLR2, TLR4), and DNA interaction without interference from the acidic tail. The protein is fully compatible with in vitro biochemical assays, cell culture studies, and receptor-ligand binding analyses.
The product is produced in E.coli from an expression plasmid coding for the human HMGB1 sequence (amino acids 1–176) without any tags or modifications, ensuring natural folding and charge distribution.

Purification: Purified. Oxidation of cysteine 106 makes the protein inactive. To avoid cysteine oxidation DTT 0.5 mM is added during protein purification.

niveau d'endotoxine: LPS-free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: The lyophilized protein should be reconstituted in distilled water, resulting in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5 mM DTT.

Buffer: The protein is lyophilized from 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5 mM DTT.
The product contains contains < 0.006% v/v of Triton X-114 due to LPS removal. Remaining traces can be removed upon request.

Stock: 4 °C/-20 °C

Stockage commentaire: 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C).

Détail du antigène

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Autre désignation: High-mobility group protein B1 (HMGB1)

Sujet: HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.

Poids moléculaire: 20.0 kDa

Pathways: Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome