HMGB1 Protein (BoxA 2D)

N° du produit ABIN7862613

Cette protéine Recombinant HMGB1 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour HMGB1 Protein (BoxA 2D) (ABIN7862613)

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Negative Control (NC)

Pureté: > 95 % It contains no nucleic acids.

Détail du produit

Attributs du protein: BoxA 2D

Fonction: BoxA 2D (negative control of BoxA wt), LPS-free

Séquence: BoxA 2D from HMGB1 consists of 89 amino acids: MGKGDPKKPR GKMSSYAFFV QTDREEHKKK HPDASVNFSE FSKKDSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKF

Attributs du produit: BoxA 2D is the mutant form of BoxA (DNA binding domains of HMGB1 protein) in which the two cysteines are replaced with aspartic acid.
It consists of 89 amino acids and has a calculated molecular mass of approximately 10.4 kDa.
BoxA 2D in migration assay cannot block the HMGB1 induced migration of fibroblasts.
It contains only trace amounts of LPS (<0.4 ng/mg protein).
BoxA 2D variant can be used as negative control of BoxA wt.

Purification: Purified

niveau d'endotoxine: LPS-free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: The lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl.

Buffer: The protein is lyophilized from 50 mM HEPES buffer, pH 7.9 and 500 mM NaCl.
The product contains contains < 0.006% v/v of Triton X-114 due to LPS removal. Remaining traces can be removed upon request.

Stock: 4 °C/-20 °C

Stockage commentaire: 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C).

Détail du antigène

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Autre désignation: High-mobility group protein B1 (HMGB1)

Sujet: HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.

Poids moléculaire: 10.4 kDa

Pathways: Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome