KIT Ligand Protein (KITLG) (full length)

N° du produit ABIN935785

Protéine Recombinant KIT Ligand exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour KIT Ligand Protein (KITLG) (full length) (ABIN935785)

Antigène: KIT Ligand (KITLG)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 98 % pure

Détail du produit

Attributs du protein: full length

Attributs du produit: Purified recombinant Human SCF protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is

Purification: Proprietary chromatographic technique

Information d'application

Indications d'application: Each Investigator should determine their own optimal working dilution for specific applications.

Commentaires:

Biological activity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is

Procédure de l'essai:

Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in sterile water not less than 100 μg/mL.

Buffer: Lyophilized from a concentrated (1 mg/mL) solution in water containing 11 mM CH3COOH.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Détail du antigène

Antigène: KIT Ligand (KITLG)

Autre désignation: SCF

Sujet: Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as steel factor or c-kit ligand. SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).
Alternative Names: SCF protein, MGF protein, Mast cell growth factor protein, Kitl protein, c-Kit Ligand protein, Steel Factor protein, KL-1 protein, MGF protein, C-kit ligand protein, SF protein, Mast Cell Growth Factor protein, Kit ligand Precursor protein, DKFZp686F2250, Stem Cell Factor protein

Poids moléculaire: 18.409 Da

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway