anti-Humain RACGAP1 anticorps pour Western Blotting

Recommended RACGAP1 Antibody

Antigène
Rac GTPase Activating Protein 1 (RACGAP1) Anticorps
  • CYK4
  • HsCYK-4
  • ID-GAP
  • MgcRacGAP
  • cyk-4
  • id-gap
  • mgcracgap
  • RACGAP1
  • MGC121578
  • AI227039
  • AI327394
  • Band25
  • GTPase
  • gtl11
  • mKIAA1478
  • wu:fb92c08
  • zgc:77839
  • Rac GTPase activating protein 1
  • rac GTPase-activating protein 1
  • Rac GTPase activating protein 1 L homeolog
  • Rac GTPase-activating protein 1
  • RACGAP1
  • racgap1
  • racgap1.L
  • racgap1.2.L
  • racgap1.2
  • Racgap1
Épitope
C-Term
33
21
17
11
8
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain
160
90
71
11
11
10
7
6
6
3
3
Hôte
Chèvre
134
18
6
2
Clonalité
Polyclonal
Conjugué
Cet anticorp RACGAP1 est non-conjugé
10
6
6
4
4
4
4
4
4
3
3
3
2
2
2
2
2
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
114
66
63
54
26
21
20
18
15
9
2
2
1
Options

Images

N° du produit ABIN184690
$ 429.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
29.452406 ABIN2856889 ICC IF IHC (p) WB Rabbit N-Term Polyclonal 0
21.357807 ABIN565414 ELISA WB Mouse IgG2b kappa AA 1-110 1G6 4
17.452406 ABIN3186646 ELISA IF IHC WB Rabbit IgG Tyr700 Polyclonal 0
13.857807 ABIN954428 EIA FACS IF IHC (p) WB Rabbit Ig Fraction AA 30-58, N-Term Polyclonal 2
13.857807 ABIN6255287 ELISA ICC IF IHC WB Rabbit IgG pSer387 Polyclonal 0
13.857807 ABIN1876486 IF IHC WB Rabbit IgG Polyclonal 0
13.857807 ABIN5867080 ELISA WB Goat Polyclonal 0
13.857807 ABIN4952348 ELISA FACS ICC IF IHC WB Goat Ig Fraction Polyclonal 0
13.594599 ABIN3182810 ELISA IF IHC WB Rabbit IgG pSer387 Polyclonal 0
13.594599 ABIN3186645 ELISA IF IHC WB Rabbit IgG N-Term Polyclonal 0
13.594599 ABIN5620153 ICC IF IHC WB Rabbit N-Term Polyclonal 0
13.594599 ABIN2150221 IF IHC ELISA WB Rabbit IgG Polyclonal 0
13.594599 ABIN6146621 IF WB Rabbit Polyclonal 0
13.594599 ABIN5622015 ICC IF IHC (p) WB Rabbit IgG Polyclonal 0
11.5 ABIN546800 IHC (p) ELISA WB Goat Polyclonal 1
10.857807 ABIN2707626 IC IF IHC WB Rabbit N-Term Polyclonal 0
10.857807 ABIN1531650 IF IHC ELISA WB Rabbit IgG AA 353-402, pSer387 Polyclonal 1
10.857807 ABIN6264604 ELISA ICC IF IHC WB Rabbit IgG Polyclonal 0
10.857807 ABIN6262125 ELISA ICC IF WB Rabbit IgG Polyclonal 0
10.857807 ABIN2957665 IC IF IHC WB Rabbit Polyclonal 0

General

Antigène Rac GTPase Activating Protein 1 (RACGAP1) Anticorps
Épitope C-Term
(33), (21), (17), (11), (8), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Humain
(160), (90), (71), (11), (11), (10), (7), (6), (6), (3), (3)
Hôte Chèvre
(134), (18), (6), (2)
Clonalité
Polyclonal
Conjugué Cet anticorp RACGAP1 est non-conjugé
(10), (6), (6), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2)
Application ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(114), (66), (63), (54), (26), (21), (20), (18), (15), (9), (2), (2), (1)
Pubmed 1 référence disponible
Fournisseur

Détail du produit anti-RACGAP1 anticorps

Détail du antigène RACGAP1 Information d'application Stockage References for anti-RACGAP1 antibody (ABIN184690) Images
Fonction RACGAP1 / MgcRacGAP
Blocking Peptide Blocking peptide for this product available: ABIN369135
Séquence GRQGNFFASP MLK
Specificité This antibody is expected to recognize all reported isoforms (NP_037409.2, NP_001306934.1, NP_001306935.1, NP_001306936.1). Reported variants represent identical protein: NP_001306928.1, NP_001119576.1, NP_037409.2, NP_001306931.1, NP_001306929.1, NP_001119575.1, NP_001306932.1, NP_001306930.1, NP_001306933.1
Homologie Human, Dog, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Classe de qualité Verified
Immunogène Peptide with sequence C-GRQGNFFASPMLK, from the C Terminus of the protein sequence according to NP_037409.2, NP_001119576.1, NP_001119575.1.
Plasmids, Primers & others

Détail du antigène RACGAP1

Détail du produit anti-RACGAP1 anticorps Information d'application Stockage References for anti-RACGAP1 antibody (ABIN184690) Images Haut de la page
Antigène
Autre désignation RACGAP1 (RACGAP1 Antibody Extrait)
Sujet RACGAP1, MgcRacGAP, Rac GTPase activating protein 1, ID-GAP, MGCRACGAP, GTPase activating protein, HsCYK-4, HsCYK-4
ID gène 29127
NCBI Accession NP_037409, NP_001306934, NP_001306935, NP_001306936
Pathways Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor

Information d'application

Détail du produit anti-RACGAP1 anticorps Détail du antigène RACGAP1 Stockage References for anti-RACGAP1 antibody (ABIN184690) Images Haut de la page
Indications d'application

DS WB Results: Approx 75 kDa band observed in lysates of cell line Jurkat, and in nuclear lysates of cell lines A431 and Jurkat (calculated MW of 71.0 kDa according to NP_037409.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.

DS IHC Results: In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration: 1 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Restrictions For Research Use only

Stockage

Détail du produit anti-RACGAP1 anticorps Détail du antigène RACGAP1 Information d'application References for anti-RACGAP1 antibody (ABIN184690) Images Haut de la page
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Minimize freezing and thawing.
Stock -20 °C
Stockage commentaire Aliquot and store at -20 °C.

References for anti-RACGAP1 antibody (ABIN184690)

Détail du produit anti-RACGAP1 anticorps Détail du antigène RACGAP1 Information d'application Stockage Images Haut de la page
Background publications

Reibel, Dorseuil, Stancou, Bertoglio, Gacon: "A hemopoietic specific gene encoding a small GTP binding protein is overexpressed during T cell activation." dans: Biochemical and biophysical research communications, Vol. 175, Issue 2, pp. 451-8, 1991

Images

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Images (Fournisseur)
Western Blotting (WB) image for anti-Rac GTPase Activating Protein 1 (RACGAP1) (C-Term) antibody (ABIN184690) ABIN184690 staining (0.2µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protei...
Immunohistochemistry (IHC) image for anti-Rac GTPase Activating Protein 1 (RACGAP1) (C-Term) antibody (ABIN184690) ABIN184690 (1µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen re...
Immunohistochemistry (IHC) image for anti-Rac GTPase Activating Protein 1 (RACGAP1) (C-Term) antibody (ABIN184690) ABIN184690 (4µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen re...
Western Blotting (WB) image for anti-Rac GTPase Activating Protein 1 (RACGAP1) (C-Term) antibody (ABIN184690) ABIN184690 (1µg/ml) staining of A431 nuclear (A), Jurkat (B), Jurkat nuclear (C) and ...
Immunofluorescence (IF) image for anti-Rac GTPase Activating Protein 1 (RACGAP1) (C-Term) antibody (ABIN184690) ABIN184690 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabi...
Flow Cytometry (FACS) image for anti-Rac GTPase Activating Protein 1 (RACGAP1) (C-Term) antibody (ABIN184690) ABIN184690 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line),...