anti-Cheval Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase anticorps pour Western Blotting

Recommended Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1) Anticorps
  • PIMT
  • C79501
  • PCM
  • PCMT
  • etID309741.20
  • fj13d06
  • pimt
  • wu:fj13d06
  • protein-L-isoaspartate (D-aspartate) O-methyltransferase
  • protein-L-isoaspartate (D-aspartate) O-methyltransferase 1
  • protein-L-isoaspartate (D-aspartate) O-methyltransferase L homeolog
  • protein-L-isoaspartate(D-aspartate)O-methyltransferase
  • PCMT1
  • Pcmt1
  • pcmt1.L
  • pcmt1
  • pcmt
Épitope
Middle Region
6
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivité
Boeuf (Vache), Chien, Chévre, Cobaye, Cheval, Humain, Souris, Lapin, Rat (Rattus), Poisson zèbre (Danio rerio)
81
32
31
1
1
1
1
1
1
1
1
1
1
Hôte
Lapin
73
9
Clonalité
Polyclonal
Conjugué
Inconjugué
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
58
21
20
19
13
12
12
8
4
4
2
2
1
1
Options
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N° du produit ABIN2773925
$ 229.00
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General

Antigène Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1) Anticorps
Épitope Middle Region
(6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivité Boeuf (Vache), Chien, Chévre, Cobaye, Cheval, Humain, Souris, Lapin, Rat (Rattus), Poisson zèbre (Danio rerio)
(81), (32), (31), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Hôte Lapin
(73), (9)
Clonalité
Polyclonal
Conjugué Inconjugué
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(58), (21), (20), (19), (13), (12), (12), (8), (4), (4), (2), (2), (1), (1)
Pubmed 1 référence disponible
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Détail du produit

Détail du antigène Information d'application Stockage Référence Images
Séquence APYDAIHVGA AAPVVPQALI DQLKPGGRLI LPVGPAGGNQ MLEQYDKLQD
Homologie Cow: 100%, Dog: 100%, Goat: 79%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%
Attributs du produit This is a rabbit polyclonal antibody against PCMT1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Immunogène The immunogen is a synthetic peptide directed towards the middle region of human PCMT1

Détail du antigène

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Antigène
Autre désignation PCMT1 (PCMT1 Antibody Extrait)
Sujet Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: PIMT
Protein Interaction Partner: RPIA, HUWE1, AIMP2, TP53, AURKA, UBC, SUMO1, NEDD8, SH3RF2, LMNA, VCAM1, ITGA4, FN1, CFTR, VKORC1, TP53BP1, SDHAF2, NANS, UBE2C, SEC24A, PPIH, ZRANB2, UCHL3, TPM4, SVIL, RRM2, RAD23A, PSMB7, PFDN2, NOTCH2, GDI1, ATP6V1B2, APOE, CUL1, CUL2, CUL3, CDK2, CAN
Protein Size: 227
Poids moléculaire 25 kDa
ID gène 5110
NCBI Accession NM_005389, NP_005380
UniProt P22061
Pathways Ribonucleoprotein Complex Subunit Organization

Information d'application

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Indications d'application Optimal working dilutions should be determined experimentally by the investigator.
Commentaires

Antigen size: 227 AA

Restrictions For Research Use only

Stockage

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles.
Stock -20 °C
Stockage commentaire For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Référence

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Background publications

Kosugi, Furuchi, Katane, Sekine, Shirasawa, Homma: "Suppression of protein l-isoaspartyl (d-aspartyl) methyltransferase results in hyperactivation of EGF-stimulated MEK-ERK signaling in cultured mammalian cells." dans: Biochemical and biophysical research communications, Vol. 371, Issue 1, pp. 22-7, 2008

Images

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Images (Fournisseur)
Western Blotting (WB) image for anti-Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1) (Middle Region) antibody (ABIN2773925) WB Suggested Anti-PCMT1 Antibody Titration: 2.5ug/ml Positive Control: HepG2 cell ...