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Lapin anti-Souris IgG1 (Heavy Chain) Anticorps (Biotin) - Preadsorbed

ELISA, WB, IHC Polyclonal IgG Biotin
N° du produit ABIN102335
  • Antigène Tous les produits IgG1
    IgG1
    Épitope
    • 41
    • 26
    • 17
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Heavy Chain
    Reactivité
    • 89
    • 26
    • 23
    • 7
    • 7
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Souris
    Hôte
    • 65
    • 41
    • 34
    • 13
    • 10
    • 6
    • 1
    Lapin
    Clonalité
    • 97
    • 60
    Polyclonal
    Conjugué
    • 46
    • 18
    • 18
    • 15
    • 11
    • 8
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    Application
    • 77
    • 57
    • 55
    • 41
    • 32
    • 21
    • 18
    • 13
    • 11
    • 10
    • 10
    • 9
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
    Specificité
    IgG1
    Attributs du produit
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Immunogène

    Immunogen: Mouse IgG1 heavy chain

    Isotype
    IgG
    Labeling Ratio
    10-20
  • Indications d'application

    Immunohistochemistry Dilution: 1:500 - 1:3,000

    Application Note: Mouse IgG1 secondary antibody conjugated to Biotin is available in a variety of formats. Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.

    ELISA Dilution: 1:250,000

    Western Blot Dilution: 1:1,000 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Aliquot to Avoid repeated freezing and thawing.
    Stock
    RT,4 °C,-20 °C
    Date de péremption
    12 months
  • Antigène
    IgG1
    Abstract
    IgG1 Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody biotin Conjugation, Rabbit Anti-Mouse IgG1 BAC Conjugated Antibody

    Background: Anti-Mouse IgG1 Biotin Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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