Souris anti-Humain IgA + IgD + IgE + IgG + IgM Anticorps

N° du produit ABIN1099692

Détail du produit

Antigène: IgA + IgD + IgE + IgG + IgM

Reactivité: Humain

Hôte: Souris

Clonalité: Polyclonal

Application: Immunofluorescence (IF), ELISA

Attributs du produit: The reactivity of the antibody is restricted to a isotype specific determinant as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic immunoglobulins.

Monoclonal antibody concentration in the solution is 1.0 mg/mL.

Physical form: Delipidated, heat inactivated, lyophilized stable ascites.

Purification: Ascites

Immunogène: Highly purified monoclonal IgG subclasses isolated from pooled human serum.

Information d'application

Indications d'application: The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly form 1:100 to 1:500, in ELISA from 1:1,000 upwards, in Western blotting 1:2,000 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: No foreign proteins added.

Conseil sur la manipulation: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not refrozen, and preferably used the same day.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Détail du antigène

Antigène: IgA + IgD + IgE + IgG + IgM

Sujet: To identify the presence of immunoglobulin classes in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting.