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Âne anti-Mouton IgG (Heavy & Light Chain) Anticorps (DyLight 594)

IA Polyclonal DyLight 594
N° du produit ABIN1110598
  • Antigène Tous les produits IgG
    IgG
    Épitope
    • 2356
    • 502
    • 411
    • 188
    • 130
    • 124
    • 94
    • 29
    • 28
    • 28
    • 17
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reactivité
    • 700
    • 680
    • 570
    • 458
    • 368
    • 239
    • 200
    • 148
    • 140
    • 132
    • 122
    • 98
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    • 94
    • 63
    • 47
    • 38
    • 27
    • 17
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    • 10
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    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouton
    Hôte
    • 1740
    • 1486
    • 539
    • 219
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Âne
    Clonalité
    • 3369
    • 196
    • 1
    Polyclonal
    Conjugué
    • 588
    • 411
    • 408
    • 370
    • 298
    • 276
    • 228
    • 121
    • 99
    • 67
    • 66
    • 62
    • 62
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    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 14
    • 10
    DyLight 594
    Application
    • 1871
    • 1798
    • 1792
    • 1538
    • 988
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 87
    • 77
    • 52
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunoassay (IA)
    Specificité
    Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG and light chains on all sheep immunoglobulins.
    Attributs du produit
    Donkey anti-Sheep IgG (H&L), DyLight594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all sheep IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase sheep IgG.
    Purification
    Affinity purification
    Immunogène
    Purified sheep IgG, whole molecule
  • Indications d'application
    1:20 - 1:2,000 for most applications
    Commentaires

    Based on immunoelectrophoresis, this antibody reacts with: heavy (gamma) chains on sheep IgG light chains on all sheep immunoglobulins.No reactivity is observed to: non-immunoglobulin sheep serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
    Conseil sur la manipulation
    Product is photosensitive and should be protected from light.
    Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
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