Chèvre anti-Souris IgG1 (Heavy Chain) Anticorps (FITC) - Preadsorbed

N° du produit ABIN1118470

Détail du produit

Antigène: IgG1

Épitope: Heavy Chain

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum.
Specificity was confirmed by ELISA at less than 0.5 % of target signal.

Réactivité croisée: Souris

Attributs du produit: FITC Conjugated Secondary Antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Mouse IgG heavy chain

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 2.8

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Mouse IgG1 FITC conjugated secondary antibody reagents are ideal for Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy as well as other antibody detection methods. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:200 - 1:500

Western Blot Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: 1:1,000 - 1:5,000

Commentaires:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Product is photosensitive and should be protected from light.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4 °C before opening. This product is stable at 4 °C as an undiluted liquid.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG1

Abstract: IgG1 Produits

Classe de substances: Antibody

Sujet:

Synonyms: Goat anti-mouse IgG1 antibody fluorescein conjugation, goat anti-mouse IgG1 (gamma 1) FITC conjugated antibody

Background: Anti-Mouse IgG1 Fluorescein Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.