Chèvre anti-Souris IgG2a (Heavy Chain) Anticorps (FITC) - Preadsorbed

N° du produit ABIN1118478

Détail du produit

Antigène: IgG2a

Épitope: Heavy Chain

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes.

Réactivité croisée: Souris

Attributs du produit: Mouse IgG2a secondary antibody is available in a variety of formats. FITC Conjugated Secondary Antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Mouse IgG2a heavy chain

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 5.2

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Mouse IgG2a secondary antibody is available in a variety of formats. FITC Conjugated Secondary Antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:200 - 1:500

Western Blot Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: 1:1,000 - 1:5,000

Commentaires:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Stockage

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.7 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Product is photosensitive and should be protected from light.
Centrifuge product if not completely clear after standing at room temperature.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Date de péremption: 12 months

Référence

Musante, Li, Kanyo, Lam, Colangelo, Cheng, Brody, Greengard, Le Novère, Nairn: "Reciprocal regulation of ARPP-16 by PKA and MAST3 kinases provides a cAMP-regulated switch in protein phosphatase 2A inhibition." dans: eLife, Vol. 6, (2018) (PubMed).

Carlyle, Kitchen, Kanyo, Voss, Pletikos, Sousa, Lam, Gerstein, Sestan, Nairn: "A multiregional proteomic survey of the postnatal human brain." dans: Nature neuroscience, Vol. 20, Issue 12, pp. 1787-1795, (2017) (PubMed).

Andrade, Musante, Horiuchi, Matsuzaki, Brody, Wu, Greengard, Taylor, Nairn: "ARPP-16 Is a Striatal-Enriched Inhibitor of Protein Phosphatase 2A Regulated by Microtubule-Associated Serine/Threonine Kinase 3 (Mast 3 Kinase)." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 37, Issue 10, pp. 2709-2722, (2017) (PubMed).

Détail du antigène

Antigène: IgG2a

Abstract: IgG2a Produits

Classe de substances: Antibody

Sujet:

Synonyms: goat anti-MOUSE IgG2a Antibody fluorescein Conjugation, goat anti-Mouse IgG2a (gamma 2a) FITC Conjugated Antibody

Background: Anti-Mouse IgG2a Fluorescein Antibody generated in goat detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.