anti-rat Ig chain monoclonal antibody recognizes a determinant on rat kappa chains and binds preferentially to the Ig-1b allotype. This antibody provides a suitable marker for virtually all immunoglobin, regardless of class since 5 % or less of rat light chains are lambda. ABIN133280 detects surface immunoglobulin of rat peripheral blood B lymphocytes and has been used in indirect binding assays for detecting rat antibodies. Applications include flow cytometry for labelling rat B lymphocytes.
Purification
Purified from ascitic fluid via Protein G Chromatography
Immunogène
Pooled rat serum IgG . Fusion Partner: P3-NSI-1-Ag4 (NS1/1) . Donor: BALB/c spleen
Optimal working dilutions should be determined experimentally by the investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.0 mg/mL
Buffer
PBS and 0.02 % NaN.
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation
Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.