Chèvre anti-Humain IgA, IgG, IgM (Fc Region) Anticorps (HRP) - Preadsorbed

N° du produit ABIN458035

Détail du produit

Antigène: IgA, IgG, IgM

Épitope: Fc Region

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificité: The reactivity of the antiserum is directed to the Fc subunits of the major isotypes of the human immuno- globulin system. No reaction is obtained with purified IgG/Fab or any non-Ig protein of human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained

Attributs du produit: Horseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to human IgG, IgA and IgM, Fc specific
Peroxidase/IgG protein molar ratio (E/P) is approximately 1.5. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized Ig-depleted human serum fractions and IgG/Fab.

Immunogène: Purified polyclonal human IgG, and IgA and IgM containing factions isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Agent conservateur: Without preservative

Conseil sur la manipulation: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Détail du antigène

Antigène: IgA, IgG, IgM

Autre désignation: IgG + IgA + IgM (IgA, IgG, IgM Produits)

Classe de substances: Antibody