Cité dans 5 publications.
Cet anticorps anti-CD14 Monoclonal Souris conjugué à Biotin (Clone MEM-15) (ABIN93972) détecte spécifiquement CD14 dans FACS et IP.
L’anticorps est réactif avec des échantillons de Humain et Non-Human Primate.
The antibody MEM-15 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages. The antibody MEM-15 also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
Purification
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Immunogène
A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Do not freeze.
Cejkova, Nemeckova, Broz, Cerna: "TLR2 and TLR4 expression on CD14(++) and CD14(+) monocyte subtypes in adult-onset autoimmune diabetes." dans: Biomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia, (2015) (PubMed).
Majer, Vlaskova, Krol, Kalina, Kubanek, Stolnaya, Dvorakova, Elleder, Sikora: "Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency." dans: Gene, Vol. 498, Issue 2, pp. 183-95, (2012) (PubMed).
Sing, Rost, Tvardovskaia, Roggenkamp, Wiedemann, Kirschning, Aepfelbacher, Heesemann: "Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression." dans: The Journal of experimental medicine, Vol. 196, Issue 8, pp. 1017-24, (2002) (PubMed).
Funda, Tucková, Farré, Iwase, Moro, Tlaskalová-Hogenová: "CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited." dans: Infection and immunity, Vol. 69, Issue 6, pp. 3772-81, (2001) (PubMed).
Bazil, Horejsí, Baudys, Kristofová, Strominger, Kostka, Hilgert: "Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen." dans: European journal of immunology, Vol. 16, Issue 12, pp. 1583-9, (1987) (PubMed).
Antigène
CD14
Autre désignation
CD14
Sujet
CD14 Molecule,CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.,LPS-receptor, LPSR, MCLR