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MEK1 anticorps (pSer221)

Cet anticorps Lapin Polyclonal détecte spécifiquement MEK1 dans WB, IHC et IF. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.Il a été cité dans 1 publication.

(1 reference)

N° du produit ABIN362167

268,40 €

Plus frais de livraison 40,00 € et TVA

Destination: France

Envoi sous 10 à 14 jours ouvrables

Aperçu rapide pour MEK1 anticorps (pSer221) (ABIN362167)

Antigène

Voir toutes MEK1 (MAP2K1) Anticorps

MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivité

Humain, Souris, Rat

Hôte

Lapin

Clonalité

Polyclonal

Conjugué

Cet anticorp MEK1 est non-conjugé

Application

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Discover our top product MAP2K1 Anticorps primaire

Épitope
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pSer221

Specificité

The antibody detects endogenous level of MEK1 only when phosphorylated at serine 221.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène

Peptide sequence around phosphorylation site of pSer221 (A-N-S (p) -F-V) derived from Human MEK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype

IgG
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody
MAP2K1 Reactivité: Humain ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 1-100) antibody
MAP2K1 Reactivité: Humain WB, IF Hôte: Lapin Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody
MAP2K1 Reactivité: Humain, Souris, Rat, Chien, Singe WB, IHC, IF, IHC (p) Hôte: Souris Monoclonal 3H3 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody
MAP2K1 Reactivité: Humain, Souris, Rat, Chien, Singe WB, IHC, IF, IHC (p) Hôte: Souris Monoclonal 2H3 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer298) antibody
KD Validated MAP2K1 Reactivité: Humain WB, ICC, FACS Hôte: Lapin Monoclonal 24GB285 unconjugated Recombinant Antibody

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody
MAP2K1 Reactivité: Humain, Souris, Rat, Chien, Singe WB, IHC, IF, IHC (p) Hôte: Souris Monoclonal 3B4 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody
KD Validated MAP2K1 Reactivité: Humain WB, FACS Hôte: Lapin Monoclonal 23GB2475 unconjugated Recombinant Antibody

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer221) antibody
MAP2K1 Reactivité: Humain WB, IHC, ELISA, IF Hôte: Lapin Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (N-Term) antibody
MAP2K1 Reactivité: Humain, Souris, Rat, Xenopus laevis WB, IHC, IF, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr292) antibody
KD Validated MAP2K1 Reactivité: Humain WB, ICC, FACS Hôte: Lapin Monoclonal 24GB305 unconjugated Recombinant Antibody

Indications d'application

Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C/-20 °C

Stockage commentaire

Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Ikari, Atomi, Kinjo, Sasaki, Sugatani: "Magnesium deprivation inhibits a MEK-ERK cascade and cell proliferation in renal epithelial Madin-Darby canine kidney cells." dans: Life sciences, Vol. 86, Issue 19-20, pp. 766-73, (2010) (PubMed).
Antigène

MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Autre désignation

MEK1

Sujet

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Poids moléculaire

45 kDa

NCBI Accession

NP_002746

UniProt

Q02750

Pathways

Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling