ZAP70 anticorps (pTyr493)
Aperçu rapide pour ZAP70 anticorps (pTyr493) (ABIN498719)
Antigène
Voir toutes ZAP70 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- pTyr493
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Specificité
- This antibody detects endogenous levels of ZAP-70 protein only when phosphorylated at Tyr493.
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Réactivité croisée (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
Purification
- Affinity chromatography
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Pureté
- > 95 % by SDS-PAGE
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anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (C-Term) antibody
ZAP70 Reactivité: Humain WB, ICC, ICFC Hôte: Souris Monoclonal ZAP-03 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibodyZAP70 Reactivité: Humain, Souris, Rat WB, IHC, ELISA, IF, ICC Hôte: Lapin Polyclonal unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 247-382) antibodyZAP70 Reactivité: Humain IHC, ELISA, FACS, IF Hôte: Souris Monoclonal ZAP70-2035 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 169-390) antibodyZAP70 Reactivité: Humain WB, ELISA, FACS Hôte: Souris Monoclonal 1F3G9 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 337-600) antibodyZAP70 Reactivité: Humain WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibodyZAP70 Reactivité: Humain, Souris, Rat WB, IHC, ELISA, IF, ICC Hôte: Lapin Polyclonal unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 1-254) antibodyZAP70 Reactivité: Humain IHC, FACS, IF Hôte: Souris Monoclonal 2F3-2 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 169-390) antibodyZAP70 Reactivité: Humain WB, ELISA Hôte: Souris Monoclonal 6D9C2 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 247-382) antibodyZAP70 Reactivité: Humain IHC, ELISA Hôte: Souris Monoclonal ZAP70-2046 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibodyZAP70 Reactivité: Humain WB, IF Hôte: Lapin Monoclonal unconjugated
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Indications d'application
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1,0 mg/mL
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Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
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Autre désignation
- ZAP70
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Sujet
- The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti alpha and beta chains, the CD3 gamma, delta and epsilon chains and a zeta-containing homodimer or heterodimer. The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR zeta chain through two Src homology (SH2) domains. This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr 292 and Tyr 319. ZAP-70 is autophosphorylated on Tyr 292, which is thought to negatively regulate ZAP-70 function in lymphocytes. Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr 319, which mediates the SH2-dependent interaction between Lck and ZAP-70.Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70
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Poids moléculaire
- approx. 70 kDa
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ID gène
- 7535
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NCBI Accession
- NP_001070
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UniProt
- P43403
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Pathways
- TCR Signaling, Ubiquitin Proteasome Pathway
Antigène
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