The antibody recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
Purification
Protein-A affinity chromatography
Pureté
> 95 % pure (by SDS-PAGE)
Immunogène
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide
Flow Cytometry. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Date de péremption
12 months
Antigène
HLA-E
(HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))
anticorps EA1.2, anticorps EA2.1, anticorps HLA-6.2, anticorps MHC, anticorps QA1, anticorps major histocompatibility complex, class I, E, anticorps HLA-E, anticorps PATR-E
Sujet
HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.2, HLAE, MHC class I antigen E, alpha chain E