Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificité
This antibody is specific for ~180k NMDAR NR2B subunit protein phosphorylated at Tyr1336. Immunolabeling of the NMDAR NR2B subunit band is blocked by lambda-phosphatase treatment.
Réactivité croisée (Details)
Species reactivity (expected):Human, Mouse and non-Human Primates. Species reactivity (tested):Rat.
Purification
Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogène
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1336 of the NR2B subunit of the rat NMDA receptor.
GRIN2B
Reactivité: Rat
WB, IHC, IF, IP, ICC, AA
Hôte: Souris
Monoclonal
S59
Atto 390
Indications d'application
Western blot: 1/1000. Immunohistochemistry: 1/400. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the antibody undiluted (in aliquots) at-20 °C.
Hicklin, Wu, Radcliffe, Freund, Goebel-Goody, Correa, Proctor, Lombroso, Browning: "Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 16, pp. 6650-5, (2011) (PubMed).
The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr1336 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002) and ischemia may also increase the phosphorylation of this site (Takagi et al., 2003).Synonyms: GRIN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NMDA Receptor 2B