Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Intracellular Staining (ICS)
Marque
BD Pharmingen™
Réactivité croisée
Humain
Attributs du produit
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results. 2. Please refer to us for technical protocols. 3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. 4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. 5. An isotype control should be used at the same concentration as the antibody of interest.
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C.
Kawamata, Du, Li, Lavau: "Notch1 perturbation of hemopoiesis involves non-cell- autonomous modifications." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 4, pp. 1738-45, (2002) (PubMed).
Anderson, Robey, Huang: "Notch signaling in lymphocyte development." dans: Current opinion in genetics & development, Vol. 11, Issue 5, pp. 554-60, (2001) (PubMed).
Kojika, Griffin: "Notch receptors and hematopoiesis." dans: Experimental hematology, Vol. 29, Issue 9, pp. 1041-52, (2001) (PubMed).
Walker, Carlson, Tan-Pertel, Weinmaster, Gasson: "The notch receptor and its ligands are selectively expressed during hematopoietic development in the mouse." dans: Stem cells (Dayton, Ohio), Vol. 19, Issue 6, pp. 543-52, (2001) (PubMed).
Huppert, Le, Schroeter, Mumm, Saxena, Milner, Kopan: "Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1." dans: Nature, Vol. 405, Issue 6789, pp. 966-70, (2000) (PubMed).
Artavanis-Tsakonas, Rand, Lake: "Notch signaling: cell fate control and signal integration in development." dans: Science (New York, N.Y.), Vol. 284, Issue 5415, pp. 770-6, (1999) (PubMed).
Milner, Bigas: "Notch as a mediator of cell fate determination in hematopoiesis: evidence and speculation." dans: Blood, Vol. 93, Issue 8, pp. 2431-48, (1999) (PubMed).
Milner, Bigas, Kopan, Brashem-Stein, Bernstein, Martin: "Inhibition of granulocytic differentiation by mNotch1." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 23, pp. 13014-9, (1996) (PubMed).
The Notch family of transmembrane receptors controls cell-fate decisions during the development of many organs in a wide variety of animal species. After binding its ligand, a Notch receptor is cleaved in its transmembrane domain, and the resulting intracellular domain dissociates from the membrane and translocates to the nucleus, where it is able to suppress the expression of lineage-specific genes by interacting with transcriptional repressors. The mN1A antibody reacts with the intracellular domain of mouse and human Notch1, but not with mouse Notch2, 3, or 4. In the mouse, Notch1 mRNA is expressed in mouse hematopoietic cells of the fetal liver and adult thymus and bone marrow. Using mAb mN1A, Notch1 is detected in CD4-CD8- (double-negative) and CD4-CD8+ thymocytes. Studies of Notch1-transgenic cells and Notch1-null mice indicate that the receptor is involved in the regulation of lymphopoiesis and myelopoiesis. The mN1A mAb does not cross-react with rat thymocytes. An alternative anti-mouse Notch1 monoclonal antibody, clone 22E5.5, specifically binds to an extracellular domain of mouse Notch1. Synonyms: Notch1, Notch 1, NOTC1, lin-12, Mis6, Motch A, mT14, N1, NICD, p300, Tan1