anti-Poulet ZAP70 anticorps pour Immunofluorescence

Recommended ZAP70 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Anticorps
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta-chain (TCR) associated protein kinase 70kDa
  • tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta-chain (TCR) associated protein kinase 70
  • zap70
  • ZAP70
  • Smp_149470
  • Zap70
Épitope
AA 468-619
79
53
49
37
28
24
23
15
13
12
10
10
10
8
8
8
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Poulet, Humain, Souris, Rat (Rattus)
580
218
142
14
11
10
8
8
6
6
4
3
1
Hôte
Souris
315
242
16
8
3
Clonalité
Monoclonal
Conjugué
Cet anticorp ZAP70 est non-conjugé
31
24
20
19
13
9
9
7
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
2
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
318
172
169
161
158
82
52
48
23
7
5
5
4
4
4
3
3
2
2
Options
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N° du produit ABIN967848
146,73 €
Plus frais de livraison 40,00 € et TVA
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
1 ABIN967849 IF IP IHC WB Mouse IgG2a AA 468-619 Connectez-vous pour afficher 29-ZAP70 Kinase 5

General

Antigène zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Anticorps
Épitope AA 468-619
(79), (53), (49), (37), (28), (24), (23), (15), (13), (12), (10), (10), (10), (8), (8), (8), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Poulet, Humain, Souris, Rat (Rattus)
(580), (218), (142), (14), (11), (10), (8), (8), (6), (6), (4), (3), (1)
Hôte Souris
(315), (242), (16), (8), (3)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp ZAP70 est non-conjugé
(31), (24), (20), (19), (13), (9), (9), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(318), (172), (169), (161), (158), (82), (52), (48), (23), (7), (5), (5), (4), (4), (4), (3), (3), (2), (2)
Pubmed 5 références disponible
Fournisseur Connectez-vous pour afficher

Détail du produit anti-ZAP70 anticorps

Détail du antigène ZAP70 Information d'application Stockage ProductDetails: References for anti-ZAP70 antibody (ABIN967848) Images
 Réactivité croisée Souris, Rat (Rattus), Poulet
Attributs du produit 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogène Human ZAP-70 Kinase aa. 468-619
Clone 29-ZAP70 Kinase
Isotype IgG2a

Détail du antigène ZAP70

Détail du produit anti-ZAP70 anticorps Information d'application Stockage ProductDetails: References for anti-ZAP70 antibody (ABIN967848) Images Haut de la page
Antigène
Autre désignation ZAP-70 (ZAP70 Antibody Extrait)
Sujet ZAP-70 is a protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 contains two SH2-like domains with the PTK domain being at the C-terminus. It appears that both ZAP-70 and Syk are recruited to the phosphorylated CD3 and zeta subunits after TCR stimulation. The src-family PTKs, lck, and fyn, seem to play a role upstream of ZAP-70 and Syk where they phosphorylate CD3 and zeta. Thus both Src- and ZAP-70/Syk-family PTKs are critical for efficient induction of tyrosine phosphoproteins in heterologous cells. The significance of ZAP-70 in mediating TCR signal transduction has been confirmed by showing that ZAP-70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP-70 kinase domain which affect the stability of the protein. The levels of lck and fyn appear to be normal in the same cells. Results of the ZAP-70 mutations in these patients include an unusual absence of peripheral CD8+ T cells and an abundance of CD4+ T cells in peripheral lymphoid organs. These results indicate that these two populations of T cells develop through utilization of different intracellular signaling pathways.
Poids moléculaire 70 kDa
Domaine de recherche Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling

Information d'application

Détail du produit anti-ZAP70 anticorps Détail du antigène ZAP70 Stockage ProductDetails: References for anti-ZAP70 antibody (ABIN967848) Images Haut de la page
Commentaires

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only

Stockage

Détail du produit anti-ZAP70 anticorps Détail du antigène ZAP70 Information d'application ProductDetails: References for anti-ZAP70 antibody (ABIN967848) Images Haut de la page
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store undiluted at -20°C.

ProductDetails: References for anti-ZAP70 antibody (ABIN967848)

Détail du produit anti-ZAP70 anticorps Détail du antigène ZAP70 Information d'application Stockage Images Haut de la page
Produit citée dans:

Michel, Attal-Bonnefoy, Mangino, Mise-Omata, Acuto: "CD28 as a molecular amplifier extending TCR ligation and signaling capabilities." dans: Immunity, Vol. 15, Issue 6, pp. 935-45, 2001

Mora, Stanley, Armistead, Chan, Boothby: "Inefficient ZAP-70 phosphorylation and decreased thymic selection in vivo result from inhibition of NF-kappaB/Rel." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5628-35, 2001

Maccalli, Pisarra, Vegetti, Sensi, Parmiani, Anichini: "Differential loss of T cell signaling molecules in metastatic melanoma patients' T lymphocyte subsets expressing distinct TCR variable regions." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 12, pp. 6912-23, 2000

Background publications

Arpaia, Shahar, Dadi, Cohen, Roifman: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." dans: Cell, Vol. 76, Issue 5, pp. 947-58, 1994

Chan, Kadlecek, Elder, Filipovich, Kuo, Iwashima, Parslow, Weiss: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." dans: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, 1994

Images

Détail du produit anti-ZAP70 anticorps Détail du antigène ZAP70 Information d'application Stockage ProductDetails: References for anti-ZAP70 antibody (ABIN967848) Haut de la page
Supplier Images
Western Blotting (WB) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 468-619) antibody (ABIN967848) Western blot analysis of ZAP-70 Kinase on a Jurkat cell lysate (Human T-cell leukemia...