anti-ClpX Caseinolytic Peptidase X Homolog (E. Coli) (CLPX) Anticorps

ATP-dependent specificity component of the Clp protease. De plus, nous expédions CLPX Protéines (3) et beaucoup plus de produits pour cette protéine.

afficher tous les anticorps Gène GeneID UniProt
CLPX 270166 Q9JHS4
CLPX 10845 O76031
CLPX 300786 Q5U2U0
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Top anti-CLPX Anticorps sur anticorps-enligne.fr

Showing 10 out of 34 products:

Catalogue No. Reactivité Hôte Conjugué Application Images Quantité Livraison Prix Détails
Boeuf (Vache) Lapin Inconjugué WB Host: Rabbit Target Name: EGFL8 Sample Type: Hela Antibody Dilution: 1.0ug/ml  CLPX is supported by BioGPS gene expression data to be expressed in HeLa Host: Rabbit Target Name: NOP56 Sample Type: MCF7 Antibody Dilution: 1.0ug/ml  CLPX is supported by BioGPS gene expression data to be expressed in MCF7 100 μL 2 to 3 Days
$319.00
Détails
Humain Lapin Inconjugué EIA, FACS, IHC (p), WB CLPX Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. CLPX antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLPX antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. 0.4 mL 6 to 8 Days
$484.00
Détails
Humain Lapin Inconjugué FACS, IHC (p), WB CLPX Antibody (C-term)  western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CLPX antibody detected the CLPX protein (arrow). CLPX Antibody (C-term)  western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the CLPX antibody detected the CLPX protein (arrow). 400 μL 10 to 11 Days
$385.00
Détails
Humain Lapin Inconjugué ELISA, FACS, ICC, IHC, IHC (fro), WB Western blot analysis of CLPX using anti-CLPX antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates.  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLPX antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLPX at approximately 69KD. The expected band size for CLPX is at 69KD. IHC analysis of CLPX using anti-CLPX antibody . CLPX was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CLPX Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg 4 to 6 Days
$280.00
Détails
Roussette (Chauve-souris) Lapin Inconjugué WB 100 μL 11 to 14 Days
$581.17
Détails
Humain Lapin Inconjugué FACS, IHC (p), WB Western blot analysis of lysate from human heart tissue lysate, using CLPX Antibody at 1:1000 at each lane. CLPX antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. 400 μL 2 to 3 Days
$515.63
Détails
Humain Lapin Inconjugué FACS   100 μg 4 to 6 Days
$290.00
Détails
Humain Lapin Inconjugué IF, IHC (p) Immunofluorescent staining of human cell line U-2 OS with CLPX polyclonal antibody  at 1-4 ug/mL dilution shows positivity in mitochondria. Immunohistochemical staining of human testis with CLPX polyclonal antibody  shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts. 100 μL 11 to 12 Days
$577.33
Détails
Humain Lapin Inconjugué FACS, IHC, ELISA, WB   200 μL 11 to 16 Days
$658.43
Détails
Humain Lapin PE FACS, IHC, ELISA, WB   200 μL 11 to 14 Days
$1,101.83
Détails

anti-CLPX Anticorps mieux référencés

  1. Human Polyclonal CLPX Primary Antibody pour FACS, IHC (p) - ABIN654723 : Gispert, Parganlija, Klinkenberg, Dröse, Wittig, Mittelbronn, Grzmil, Koob, Hamann, Walter, Büchel, Adler, Hrabé de Angelis, Busch, Zell, Reichert, Brandt, Osiewacz, Jendrach, Auburger: Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors. dans Human molecular genetics 2013 (PubMed)

Plus d’anticorps contre CLPX partenaires d’interaction

Mouse (Murine) ClpX Caseinolytic Peptidase X Homolog (E. Coli) (CLPX) interaction partners

  1. results illustrate that ClpX overexpression is a good and simple model to study the underlying mechanisms of the UPRmt in mammalian cells.

  2. Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors.

Human ClpX Caseinolytic Peptidase X Homolog (E. Coli) (CLPX) interaction partners

  1. The mutation in CLPX inactivates its ATPase activity, resulting in coassembly of mutant and WT protomers to form an enzyme with reduced activity. The presence of low-activity CLPX increases the posttranslational stability of ALAS, causing increased ALAS protein and ALA levels, leading to abnormal accumulation of PPIX.

  2. Data suggest that tumors exploit ClpXP-directed proteostasis to maintain mitochondrial bioenergetics, buffer oxidative stress, and enable metastatic competence.

  3. results illustrate that ClpX overexpression is a good and simple model to study the underlying mechanisms of the UPRmt in mammalian cells.

  4. Optical trapping to assay single-molecule ClpXP unfolding and translocation of substrates consisting of domains with varying stabilities and sequences; find that ClpXP unfolds most domains by a single pathway, with kinetics that depend on the native fold and structural stability.

  5. Walker B mutation in human CLPX exhibits improved interaction with the model unfolded substrate casein and several putative physiological substrates in vitro.

  6. human ClpX, a novel mtDNA regulator, maintains mtDNA nucleoid distribution through TFAM function as a chaperone rather than as a protease and its involvement in mtDNA segregation.

  7. hClpX can regulate the appearance of hClpP peptidase activity in mitochondria and might affect the nature of the degradation products released during ATP-dependent proteolytic cycles

  8. Results reveal that the ssrA tag interacts with different loops that form the top, middle, and lower portions of the central channel of the ClpX hexamer.

CLPX profil antigène

Profil protéine

ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP (By similarity).

Gene names and symbols associated with CLPX

  • caseinolytic mitochondrial matrix peptidase chaperone subunit (Clpx) anticorps
  • caseinolytic mitochondrial matrix peptidase chaperone subunit (CLPX) anticorps
  • AU014732 anticorps
  • E330029I21 anticorps

Protein level used designations for CLPX

ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial , caseinolytic protease X , ClpX caseinolytic protease X homolog , energy-dependent regulator of proteolysis , caseinolytic peptidase X

GENE ID SPECIES
270166 Mus musculus
10845 Homo sapiens
300786 Rattus norvegicus
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