anti-SET Domain, Bifurcated 1 (SETDB1) Anticorps

SETDB1 encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. De plus, nous expédions SETDB1 Protéines (7) et et beaucoup plus de produits pour cette protéine.

afficher tous les anticorps Gène GeneID UniProt
SETDB1 689883  
SETDB1 9869 Q15047
SETDB1 84505  
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Top anti-SETDB1 Anticorps sur anticorps-enligne.fr

Showing 10 out of 106 products:

Catalogue No. Reactivité Hôte Conjugué Application Images Quantité Livraison Prix Détails
Humain Lapin Inconjugué ELISA, WB Western blot analysis is shown using  Affinity Purified anti-ESET antibody to detect human ESET present in a 293 whole cell lysate.   ~30µg of lysate was loaded per lane for SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected.  Peptide competition (not shown) blocks staining of this band.  The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using800 labeled Goat-a-Rabbit IgG [H&L]  diluted 1:5,000 for 45 min. 800 fluorescence image was captured using the Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results. 100 μg 5 to 7 Days
$598.50
Détails
Humain Lapin Inconjugué WB Western blot analysis of anti-SETDB1 Pab(ABIN387924) in Ramos cell line lysate (35 µg/lane). SETDB1(arrow) was detected using the purified polyclonal antibody (1:60 dilution). Western blot analysis of SETDB1(arrow) using rabbit polyclonal SETDB1 Antibody (C-term) (ABIN387924). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the SETDB1 gene (Lane 2) (Origene Technologies). 400 μL 10 to 11 Days
$385.00
Détails
Humain Lapin Inconjugué EIA, WB 0.4 mL 6 to 8 Days
$484.00
Détails
Boeuf (Vache) Lapin Inconjugué WB Host:  Rabbit  Target Name:  SETDB1  Sample Type:  HT1080 Whole cell lysates  Antibody Dilution:  1.0ug/ml 100 μL 2 to 3 Days
$289.00
Détails
Humain Lapin Inconjugué IP, IHC, WB 100 μg 2 to 3 Days
$335.00
Détails
Humain Lapin Inconjugué ELISA, IHC, WB Western blot analysis of KMT1E using anti-KMT1E antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: human placenta tissue lysate, Lane 2: rat brain tissue lysate, Lane 3: rat ovary tissue lysate, Lane 4: mouse brain tissue lysate.  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KMT1E antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KMT1E at approximately 180KD. The expected band size for KMT1E is at 143KD. IHC analysis of KMT1E/SETDB1 using anti-KMT1E/SETDB1 antibody . KMT1E/SETDB1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-KMT1E/SETDB1 Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg 4 to 6 Days
$240.00
Détails
Humain Souris Inconjugué ELISA, WB Figure 1: Western blot analysis using SETDB1 mouse mAb against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5) and F9 (6) cell lysate. Dilution: 1/500 - 1/2000 100 μL 8 to 11 Days
$491.79
Détails
Humain Souris Inconjugué ELISA, ICC, WB Figure 1: Western blot analysis using SETDB1 mouse mAb against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5), F9 (6), RAW246.7 (7) and Cos7 (8) cell lysate. Dilution: 1/500 - 1/2000 Figure 2: Immunofluorescence analysis of LOVO cells using SETDB1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Dilution: 1/200 - 1/1000 100 μL 8 to 11 Days
$491.79
Détails
Humain Lapin Inconjugué ELISA, IHC, IHC (p), WB Human Testis (formalin-fixed, paraffin-embedded) stained with SETDB1 antibody ABIN214601 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody ABIN481713, alkaline phosphatase-streptavidin and chromogen. Anti-SETDB1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. 50 μg 11 to 14 Days
$484.00
Détails
Humain Lapin Inconjugué EIA, WB   0.1 mg 4 to 8 Days
$632.50
Détails

anti-SETDB1 Anticorps mieux référencés

  1. Human Polyclonal SETDB1 Primary Antibody pour ELISA, WB - ABIN129618 : Harte, Wu, Carrasquillo, Matera: Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids. dans Cytogenetics and cell genetics 1999 (PubMed)
    Show all 4 Pubmed References

  2. Human Polyclonal SETDB1 Primary Antibody pour ELISA, IHC - ABIN4353068 : Schultz, Ayyanathan, Negorev, Maul, Rauscher: SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins. dans Genes & development 2002 (PubMed)
    Show all 3 Pubmed References

  3. Human Polyclonal SETDB1 Primary Antibody pour ICC, IF - ABIN4353070 : Ceol, Houvras, Jane-Valbuena, Bilodeau, Orlando, Battisti, Fritsch, Lin, Hollmann, Ferré, Bourque, Burke, Turner, Uong, Johnson, Beroukhim, Mermel, Loda, Ait-Si-Ali, Garraway, Young, Zon: The histone methyltransferase SETDB1 is recurrently amplified in melanoma and accelerates its onset. dans Nature 2011 (PubMed)
    Show all 2 Pubmed References

  4. Human Monoclonal SETDB1 Primary Antibody pour ELISA, WB - ABIN967016 : Rosendorff, Sakakibara, Lu, Kieff, Xuan, DiBacco, Shi, Shi, Gill: NXP-2 association with SUMO-2 depends on lysines required for transcriptional repression. dans Proceedings of the National Academy of Sciences of the United States of America 2006 (PubMed)
    Show all 3 Pubmed References

  5. Human Polyclonal SETDB1 Primary Antibody pour IF (p), IHC (p) - ABIN1387645 : Marco, Kisliouk, Tabachnik, Weller, Meiri et al.: DNA CpG Methylation (5-Methylcytosine) and Its Derivative (5-Hydroxymethylcytosine) Alter Histone Posttranslational Modifications at the Pomc Promoter, Affecting the Impact of Perinatal Diet on ... dans Diabetes 2016 (PubMed)

  6. Human Monoclonal SETDB1 Primary Antibody pour ICC, ELISA - ABIN969394 : Gevaert, Staes, Van Damme, De Groot, Hugelier, Demol, Martens, Goethals, Vandekerckhove: Global phosphoproteome analysis on human HepG2 hepatocytes using reversed-phase diagonal LC. dans Proteomics 2005 (PubMed)
    Show all 2 Pubmed References

  7. Human Polyclonal SETDB1 Primary Antibody pour BP, ChIP - ABIN4353071 : Skuland, Ovrevik, Låg, Schwarze, Refsnes: Silica nanoparticles induce cytokine responses in lung epithelial cells through activation of a p38/TACE/TGF-α/EGFR-pathway and NF-κΒ signalling. dans Toxicology and applied pharmacology 2014 (PubMed)

Plus d’anticorps contre SETDB1 partenaires d’interaction

Cow (Bovine) SET Domain, Bifurcated 1 (SETDB1) interaction partners

  1. SETDB1 is essential for the earliest events in lineage specification in the bovine blastocyst.

Pig (Porcine) SET Domain, Bifurcated 1 (SETDB1) interaction partners

  1. observations suggested that SET domain bifurcated 1 histone-lysine N-methyltransferase (SETDB1) is a novel epigenetic regulator of porcine male germ cells, and contributes to the maintenance of gonocyte survival in pigs

Human SET Domain, Bifurcated 1 (SETDB1) interaction partners

  1. SETDB1 expression is the common target of the miR-29 family members in non-small cell lung cancer.TP53 represses the expression of SETDB1 via increasing miR-29s expression in non-small cell lung cancer.

  2. Gene network analysis showed that SMAD7 expression is regulated by SETDB1 levels, indicating that up-regulation of SMAD7 by SETDB1 knockdown inhibited BRC metastasis.

  3. Although these genes are normally expressed at low amounts in hESCs, HTT knockdown (KD) reduces their induction during neural differentiation. Notably, mutant expanded polyglutamine repeats in HTT diminish its interaction with ATF7IP-SETDB1 complex and trigger H3K9me3 in HD-iPSCs.

  4. Findings indicate complicated layers of serine/threonine kinase Akt (Akt) activation regulation coordinated by SET domain, bifurcated 1 protein (SETDB1)-mediated Akt K64 methylation.

  5. Data suggest that targeting SET domain, bifurcated 1 protein (SETDB1) signalling could be a potential therapeutic strategy for combatting hyperactive AKT serine/threonine kinase 1 (AKT)-driven cancers.

  6. Smad3-mediated recruitment of SETDB1 controls Snail1 expression and epithelial-mesenchymal transition in ductal breast carcinoma.

  7. Study in hepatocellular carcinoma (HCC) cells proves that SETDB1 promotes the proliferation and migration of cells by forming SETDB1-Tiam1 compounds. SETDB1-Tiam1 compounds were involved in a novel pathway, which regulated epigenetic modification of gene expression in HCC patients samples.

  8. SETDB1 loss results in decompaction of the Xi chromosome partly through reactivation of an enhancer in the IL1RAPL1 gene.

  9. SETDB1 knockdown might suppress breast cancer progression at least partly by miR-381-3p-related regulation, providing a novel prospect in breast cancer therapy.

  10. Enhancer of Zeste Homolog 2 (EZH2), SET domain, bifurcated 1 protein (SETDB1), lysine-specific histone demethylase 1 (LSD1), histone H3 methylation (H3K9me3 and H3K27me3) expression are altered in colorectal cancer (CRC) and may play a role in colorectal carcinogenesis.

  11. Increased expression of SETDB1 may predict poor overall survival.

  12. SETDB1 is enriched at H3K9me3 regions and K9me3/K14ac is enriched at SETDB1 binding sites overlapping with LINE elements, suggesting that recruitment of the SETDB1 complex to K14ac/K9me regions has a role in silencing of active genomic regions.

  13. We identified a list of thirty genes repressed by DeltaNp63 in a SETDB1-dependent manner, whose expression is positively correlated to survival of breast cancer patients. These results suggest that p63 and SETDB1 expression, together with the repressed genes, may have diagnostic and prognostic potential

  14. these data identify a critical functional role for ATF7IP in heterochromatin formation by regulating SETDB1 abundance in the nucleus.

  15. SETDB1 triggers silencing of retrotransposons to inhibit the interferon response in acute myeloid leukemia cells.

  16. analysis of a 1q21.3 deletion encompassing SETDB1 that provides further support for the role of chromatin modifiers in the etiology of autism spectrum disorder

  17. SETDB1 protein expression was significantly associated with poor survival and was related to TNM stage.

  18. SETDB1, a major histone H3K9 methyltransferase is monoubiquitinated at the evolutionarily conserved lysine-867 in its SET-Insertion domain. This ubiquitination is directly catalyzed by UBE2E family of E2 enzymes in an E3-independent manner while the conjugated-ubiquitin (Ub) is protected from active deubiquitination.

  19. These results suggest that the ubiquitination of SETDB1 at lysine 867 controls the expression of its target gene by activating its H3K9 methyltransferase activity.

  20. Authors observed several complementary mechanisms contributing to the upregulation of SETDB1 in HCC cells. Besides copy number gains at the SETDB1 gene locus at chromosome 1q21 enhanced SETDB1 transcription mediated by the transcription factor SP1 could be detected.

Mouse (Murine) SET Domain, Bifurcated 1 (SETDB1) interaction partners

  1. the localization of SETDB1 to Dppa2, Otx2 and Utf1, and subsequent repression of their expression, are crucial for primordial germ cell determination by ensuring BMP4 signaling.

  2. MAX associated with DNA methyltransferases (DNMTs) and the histone methyltransferase SETDB1 cooperatively control germ cell-related gene expression in embryonic stem cells.

  3. SETDB1 is essential for sex chromosome remodeling and silencing during male meiosis.

  4. his suggests that setdb1 is a key chromatin modifier that modulates repressive chromatin. Conclusion These results suggest that there exist extensive mechanisms of chromatin modifications for homeostatic balance of chromatin acetylation and deconstruction of repressive chromatin during adipocyte differentiation

  5. Study suggests a more general role for Setdb1 in endogenous retroviruses silencing, which provides an additional layer of epigenetic silencing through the H3K9me3 modification.

  6. We characterized two factors, MORC2A and RESF1, of which RESF1 is a novel molecule in retroelement silencing. Although both factors are recruited to repress provirus, their roles in repression are different. MORC2A appears to function dependent on repressive epigenetic modifications, while RESF1 regulates repressive epigenetic modifications associated with SETDB1

  7. Results demonstrate a novel and unexpected role for SETDB1 in protecting IAPs from TET2-dependent histone arginine demethylation.

  8. Data show that SET domain bifurcated 1 histone-lysine N-methyltransferase (Setdb1) in macrophages suppresses Toll-like receptor 4 (TLR4)-mediated expression of proinflammatory cytokines.

  9. Results identify Setdb1 as a paternal effect gene in the mouse and suggest that epigenetic inheritance may be more likely in individuals with altered levels of epigenetic modifiers.

  10. Setdb1 regulates PTEN/AKT/FOXO1 pathway to inhibit Spermatogonial stem cells apoptosis.

  11. the SETDB1 repressor complex shields neuronal genomes from excess CTCF genome organizer binding and is critically required for structural maintenance of the protocadherin locus topologically associated domain

  12. The results identify Setdb1 as a newly discovered meiotic and embryonic competence factor safeguarding genome integrity at the onset of life.

  13. SETDB1, a major histone H3K9 methyltransferase is monoubiquitinated at the evolutionarily conserved lysine-867 in its SET-Insertion domain. This ubiquitination is directly catalyzed by UBE2E family of E2 enzymes in an E3-independent manner while the conjugated-ubiquitin (Ub) is protected from active deubiquitination.

  14. study identifed ESET as first trimethylated H3K9 histone methyltransferase playing a crucial role in T cell development

  15. Setdb1 maintains hematopoietic stem and progenitor cells by restricting the ectopic activation of nonhematopoietic genes.

  16. SETDB1 maintains silencing of intracisternal A particle, but in the absence of DNMT1, prolonged binding of NP95 to hemimethylated DNA transiently disrupts SETDB1-dependent H3K9me3 deposition.

  17. Deletion of Setdb1 led to enlarged Meckel's cartilage. Chondrocytes from the Meckel's cartilage of Setdb1 mutant showed increased in size.

  18. Setdb1 is a maternal-effect gene that controls meiotic progression and is essential for early embryogenesis. Setdb1 is an important regulator of Cdc14b.

  19. The currently identified reciprocal action between SETDB1 and PRC2 reveals a novel mechanism underlying ES cell pluripotency and differentiation regulation.

  20. regulates cancer cell growth via methylation of p53

SETDB1 profil antigène

Profil protéine

This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.

Gene names and symbols associated with SETDB1

  • SET domain, bifurcated 1 (Setdb1) anticorps
  • myeloid/lymphoid or mixed-lineage leukemia; translocated to, 11 (MLLT11) anticorps
  • SET domain bifurcated 1 (SETDB1) anticorps
  • histone-lysine N-methyltransferase SETDB1 (LOC100120014) anticorps
  • histone-lysine N-methyltransferase SETDB1 (LOC100461671) anticorps
  • SET domain, bifurcated 1a (setdb1a) anticorps
  • SET domain bifurcated 1 L homeolog (setdb1.L) anticorps
  • AU022152 anticorps
  • ESET anticorps
  • fc07d03 anticorps
  • H3-K9-HMTase4 anticorps
  • KG1T anticorps
  • KMT1E anticorps
  • mKIAA0067 anticorps
  • NV10069 anticorps
  • SETDB1 anticorps
  • si:ch211-81a5.6 anticorps
  • TDRD21 anticorps
  • wu:fc07d03 anticorps

Protein level used designations for SETDB1

histone-lysine N-methyltransferase SETDB1 , SET domain, bifurcated 1 , histone-lysine N-methyltransferase SETDB1-like , histone-lysine N-methyltransferase SETDB1-A , ERG-associated protein with SET domain , ERG-associated protein with a SET domain, ESET , H3-K9-HMTase 4 , histone H3-K9 methyltransferase 4 , histone-lysine N-methyltransferase, H3lysine-9 specific 4 , lysine N-methyltransferase 1E , tudor domain containing 21 , SET domain ERG-associated histone methyltransferase

GENE ID SPECIES
689883 Rattus norvegicus
395847 Gallus gallus
469481 Pan troglodytes
716141 Macaca mulatta
100120014 Nasonia vitripennis
100138100 Bos taurus
100157920 Sus scrofa
100411734 Callithrix jacchus
100461671 Pongo abelii
100482250 Ailuropoda melanoleuca
553292 Danio rerio
9869 Homo sapiens
84505 Mus musculus
432147 Xenopus laevis
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