EGFR anticorps (PE)

N° du produit ABIN1027691

L’anticorps Souris Monoclonal anti-EGFR a été validé pour FACS. Il convient pour détecter EGFR dans des échantillons de Humain et Cheval. Il y a 4+ publications disponibles.

Aperçu rapide pour EGFR anticorps (PE) (ABIN1027691)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain, Cheval

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGFR est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Clone: EGFR1

Détail du produit anti-EGFR anticorps (PE)

Fonction: Anti-EGFR PE

Specificité: The mouse monoclonal antibody EGFR1 reacts with extracellular domain of human protein kinase EGFR (ErbB1 / HER1), epitope within amino acids 6-273.

Aucune reactivité croisée: Souris, Rat

Réactivité croisée (Details): Human, Equine (Horse)

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: Human epidermoid carcinoma line A431

Isotype: IgG2b kappa

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-EGFR (PE) (ABIN1027691)

Rebetz, Tian, Persson, Widegren, Salford, Englund, Gisselsson, Fan: "Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma." dans: PLoS ONE, Vol. 3, Issue 4, pp. e1936, (2008) (PubMed).

Fortunel, Hatzfeld, Rosemary, Ferraris, Monier, Haydont, Longuet, Brethon, Lim, Castiel, Schmidt, Hatzfeld: "Long-term expansion of human functional epidermal precursor cells: promotion of extensive amplification by low TGF-beta1 concentrations." dans: Journal of cell science, Vol. 116, Issue Pt 19, pp. 4043-52, (2003) (PubMed).

Aigner, Juhl, Malerczyk, Tkybusch, Benz, Czubayko: "Expression of a truncated 100 kDa HER2 splice variant acts as an endogenous inhibitor of tumour cell proliferation." dans: Oncogene, Vol. 20, Issue 17, pp. 2101-11, (2001) (PubMed).

Akiyama, Smith, Shimizu: "Changing patterns of localization of putative stem cells in developing human hair follicles." dans: The Journal of investigative dermatology, Vol. 114, Issue 2, pp. 321-7, (2000) (PubMed).

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR

Sujet: Epidermal growth factor receptor,The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.,EGF-R, Her1, ERBB1, PIG61, NISBD2

ID gène: 1956

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins