PSMA anticorps

N° du produit ABIN1042485

Détail du produit anti-PSMA anticorps

Antigène: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PSMA est non-conjugé

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: 107.1A4 recognizes an antigen, which appeared to be distinct from those previously described and shows specific tumor targeting in preliminary in vivo studies. MAb 107-1A4 is suggested to recognize a distinct conformational and unique epitope in the extracellular domain of PSMA. PSMA is a type II membrane glycoprotein of 100kDa that has a short intracellular N-terminal domain (residues 1 to 18), a transmembrane region (residues 19 to 43) and a large extracellular domain consisting of residues 44 to 750.

Attributs du produit: Membrane,Tumour marker

Purification: Supernatant

Clone: 107-1A4

Isotype: IgG1

Information d'application

Indications d'application: MAb 107-1A4 could not be used in Western blot, is useful for immunohistochemistry on frozen and paraffin-embedded tissues, immunocytochemistry, flow cytometry and ELISA. Optimal antibody dilution should be determined by titration, recommended range is 1:100 – 1:1000 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Commentaires:

107.1A4 is a mouse monoclonal IgG1/k antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from an immunized mouse, using a two-phase immunization protocol involving the prostate cancer cell line LNCaP.

Restrictions: For Research Use only

Stockage

Buffer: 0.09% sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-PSMA (ABIN1042485)

Brown, Wegner, Wang, Buhler, Arfman, Lange, Vessella: "A novel monoclonal antibody 107-1A4 with high prostate specificity: generation, characterization of antigen expression, and targeting of human prostate cancer xenografts." dans: Prostate cancer and prostatic diseases, Vol. 1, Issue 4, pp. 208-215, (2002) (PubMed).

Wang, Diamond, Hass, Sokoloff, Vessella: "Identification of prostate specific membrane antigen (PSMA) as the target of monoclonal antibody 107-1A4 by proteinchip; array, surface-enhanced laser desorption/ionization (SELDI) technology." dans: International journal of cancer. Journal international du cancer, Vol. 92, Issue 6, pp. 871-6, (2001) (PubMed).

Détails sur PSMA

Antigène: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Autre désignation: PSMA (FOLH1 Produits)

Synonymes: anticorps FGCP, anticorps FOLH, anticorps GCP2, anticorps GCPII, anticorps NAALAD1, anticorps NAALAdase, anticorps PSM, anticorps PSMA, anticorps mGCP, anticorps mopsm, anticorps Naalad, anticorps putative N-acetylated-alpha-linked acidic dipeptidase, anticorps folate hydrolase 1B, anticorps folate hydrolase 1, anticorps folate hydrolase (prostate-specific membrane antigen) 1, anticorps LOC451185, anticorps FOLH1B, anticorps FOLH1, anticorps Folh1

Sujet: Prostate specific antigen (PSA) is the most widely used marker of prostate cancer. Immunoassays for PSA using monoclonal (MAb) or polyclonal antibodies have clinical applications, such as monitoring and early detection of prostate cancer. However, PSA is not a perfect tumor marker because serum levels often are elevated in men with benign prostatic hyperplasia, prostatitis and other nonmalignant disorders and also because levels are not always elevated in individuals with early prostate cancer. 107-1A4 is a new prostate cancer-reactive MAb which can identify additional proteins that could be of value for the diagnosis and treatment of prostate cancer. It shows selective reactivity to prostate epithelial membrane antigens.