VEGF anticorps (Biotin)

N° du produit ABIN1043812

Détail du produit anti-VEGF anticorps (Biotin)

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VEGF est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Specificité: Biotin Conjugated VEGF Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human VEGF-165 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Attributs du produit: VEGF (Vascular Endothelial Growth Factor A) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated. The sequence of this isoform differs from the canonical sequence as follows: 141-141: K -> N and 142-182: missing. This isoform is often found as a disulfide linked homodimer.

Immunogène: VEGF Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.
Immunogen Type: RecombinantProtein

Isotype: IgG

Information d'application

Indications d'application: VEGF conjugated antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 22 kDa in size corresponding to monomeric human VEGF-165 protein by western blotting in the appropriate cell lysate or extract.

Commentaires:

Gene Name: VEGFA

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Références pour anticorps anti-VEGF (Biotin) (ABIN1043812)

Rigopoulos, Tsiambas, Lazaris, Kavantzas, Papazachariou, Kravvaritis, Tsounis, Koliopoulou, Athanasiou, Karameris, Manaios, Sergentanis, Patsouris: "Deregulation of EGFR/VEGF/HIF-1a signaling pathway in colon adenocarcinoma based on tissue microarrays analysis." dans: Journal of B.U.ON. : official journal of the Balkan Union of Oncology, Vol. 15, Issue 1, pp. 107-15, (2010) (PubMed).

Sun, Hu, Huang, Chu, Zhang, She, Chen: "Brain-derived neurotrophic factor induces proliferation, migration, and VEGF secretion in human multiple myeloma cells via activation of MEK-ERK and PI3K/AKT signaling." dans: Tumour biology, Vol. 31, Issue 2, pp. 121-8, (2010) (PubMed).

Huez, Bornes, Bresson, Créancier, Prats: "New vascular endothelial growth factor isoform generated by internal ribosome entry site-driven CUG translation initiation." dans: Molecular endocrinology (Baltimore, Md.), Vol. 15, Issue 12, pp. 2197-210, (2001) (PubMed).

Détails sur VEGF

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Autre désignation: VEGF (VEGF Produits)

Synonymes: anticorps MVCD1, anticorps VEGF, anticorps VPF, anticorps Vegf, anticorps Vegf120, anticorps Vegf164, anticorps Vegf188, anticorps Vpf, anticorps VEGF-A, anticorps VEGF164, anticorps eVEGF120, anticorps eVEGF164, anticorps vegf-a, anticorps vegf, anticorps vascular endothelial growth factor A, anticorps vascular endothelial growth factor A L homeolog, anticorps vascular endothelial growth factor, anticorps vascular endothelial growth factor precursor, anticorps VEGFA, anticorps Vegfa, anticorps vegfa.L, anticorps VEGF, anticorps vegf

Sujet: VEGF (Vascular Endothelial Growth Factor A) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated. The sequence of this isoform differs from the canonical sequence as follows: 141-141: K → N and 142-182: missing. This isoform is often found as a disulfide linked homodimer.
Synonyms: Vascular endothelial growth factor A; VEGF-A; VEGF-165, VEGF165, VEGF isoform L, Vascular permeability factor; VPF

ID gène: 7422

NCBI Accession: NP_001165097