Galanin Receptor 1 anticorps (C-Term)
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- Antigène Voir toutes Galanin Receptor 1 (GALR1) Anticorps
- Galanin Receptor 1 (GALR1)
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Épitope
- C-Term
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Galanin Receptor 1 est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human GALR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Gorilla (100%) Human, Chimpanzee, Orangutan, Gibbon (95%) Monkey (89%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 20 amino acid peptide from C-terminus of human GALR1. Percent identity with other species by BLAST analysis: Gorilla (100%), Human, Chimpanzee, Orangutan, Gibbon (95%), Monkey (89%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product GALR1 Anticorps primaire
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- Indications d'application
- Approved: IHC, IHC-P (7 μg/mL)
- Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- Galanin Receptor 1 (GALR1)
- Autre désignation
- GALR1 / Galanin Receptor 1 (GALR1 Produits)
- Synonymes
- anticorps GALNR, anticorps GALNR1, anticorps GALR1, anticorps GALR, anticorps Galnr1, anticorps galanin receptor 1, anticorps transcription regulator. LacI family, galactose related, anticorps LacI family transcriptional regulatory protein, anticorps galactose operon repressor, anticorps GALR1, anticorps galr1, anticorps galR, anticorps galR1, anticorps Galr1, anticorps LOC100037697
- Sujet
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Name/Gene ID: GALR1
Subfamily: Galanin
Family: GPCR
Synonyms: GALR1, Galanin receptor type 1, Galanin receptor 1, Galanin receptor galr1, Galanin-1 receptor, GALNR1, Gal receptor 1, Gal-r1, Galn1r, Gal-1 receptor, GAL1-R, Galanin r1 receptor, GALNR, GALR-1, Hugal 1-r - ID gène
- 2587
- Pathways
- cAMP Metabolic Process
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