MC3R anticorps (C-Term)

N° du produit ABIN1049045

Détail du produit anti-MC3R anticorps

Antigène: MC3R (Melanocortin 3 Receptor (MC3R))

Épitope: C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MC3R est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human MC3 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MC5R (78 % ).

Homologie: Percent identity with other species by BLAST analysis: Human (100%) Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Pig (94%) Bat, Rabbit, Platypus (89%) Opossum, Turkey, Chicken, Xenopus, Trout (83%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 18 amino acid peptide from C-Terminus of human MC3 Receptor. Percent identity with other species by BLAST analysis: Human (100%), Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Pig (94%), Bat, Rabbit, Platypus (89%), Opossum, Turkey, Chicken, Xenopus, Trout (83%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 - 12 μg/mL)

Usage: Immunohistochemistry with formalin-fixed paraffin-embedded tissues requires pretreatment with Proteinase K.

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur MC3R

Antigène: MC3R (Melanocortin 3 Receptor (MC3R))

Autre désignation: MC3R / MC3 Receptor (MC3R Produits)

Synonymes: anticorps zgc:194235, anticorps MC3-R, anticorps BMIQ9, anticorps MC3, anticorps OB20, anticorps OQTL, anticorps melanocortin 3 receptor, anticorps mc3r, anticorps MC3R, anticorps Mc3r

Sujet: Name/Gene ID: MC3R
Subfamily: Melanocortin
Family: GPCR

Synonyms: MC3R, BMIQ9, MC3 receptor, Melanocortin receptor 3, OB20, MC3, MC3-R, Melanocortin 3 receptor, OQTL

ID gène: 4159

Pathways: cAMP Metabolic Process