P2RY8 anticorps (C-Term)
-
- Antigène Voir toutes P2RY8 Anticorps
- P2RY8 (Purinergic Receptor P2Y, G-Protein Coupled, 8 (P2RY8))
-
Épitope
- C-Term
-
Reactivité
- Humain
-
Hôte
- Lapin
-
Clonalité
- Polyclonal
-
Conjugué
- Cet anticorp P2RY8 est non-conjugé
-
Application
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- Human P2RY8 / P2Y8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human (100%) Gorilla (95%).
- Purification
- Immunoaffinity Chromatography
- Immunogène
- Synthetic 20 amino acid peptide from C-terminus of human P2RY8 / P2Y8.
- Top Product
- Discover our top product P2RY8 Anticorps primaire
-
-
- Commentaires
-
This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.
- Procédure de l'essai
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
-
- Concentration
- Lot specific
- Buffer
- PBS, 0.1% sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C/-80 °C
- Stockage commentaire
- Long term: -70°C, Short term: +4°C
-
- Antigène
- P2RY8 (Purinergic Receptor P2Y, G-Protein Coupled, 8 (P2RY8))
- Autre désignation
- P2RY8 / P2Y8 (P2RY8 Produits)
- Synonymes
- anticorps P2Y8, anticorps P2Y purinoceptor 8, anticorps P2Y receptor family member 8, anticorps purinergic receptor P2Y8, anticorps p2ry8, anticorps P2RY8
- Sujet
- Standard Gene Symbol: P2RY8, Gene Family: GPCR, Gene Subfamily: Orphan-A, Synonyms: P2RY8, p2RY8, p2Y purinoceptor 8, p2Y8
- ID gène
- 286530
- UniProt
- Q86VZ1
-