SLC39A14 anticorps (Internal Region)

N° du produit ABIN1049337

Détail du produit anti-SLC39A14 anticorps

Antigène: SLC39A14 (Solute Carrier Family 39 (Zinc Transporter), Member 14 (SLC39A14))

Épitope: Internal Region

Reactivité: Humain, Porc, Roussette (Chauve-souris), Chien, Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC39A14 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human SLC39A14. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Panda, Dog, Bat, Horse, Pig (100%) Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Bovine (94%) Elephant (88%) Rat, Rabbit (81%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from internal region of human SLC39A14. Percent identity with other species by BLAST analysis: Human, Gorilla, Panda, Dog, Bat, Horse, Pig (100%), Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Bovine (94%), Elephant (88%), Rat, Rabbit (81%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (15 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur SLC39A14

Antigène: SLC39A14 (Solute Carrier Family 39 (Zinc Transporter), Member 14 (SLC39A14))

Autre désignation: SLC39A14 / ZIP14 (SLC39A14 Produits)

Synonymes: anticorps LZT-Hs4, anticorps NET34, anticorps ZIP14, anticorps cig19, anticorps BC021530, anticorps G630015O18Rik, anticorps Zip14, anticorps fad123, anticorps solute carrier family 39 member 14, anticorps solute carrier family 39 (zinc transporter), member 14, anticorps SLC39A14, anticorps Slc39a14

Sujet: Name/Gene ID: SLC39A14
Subfamily: Zinc-iron transporter
Family: Transporter

Synonyms: SLC39A14, KIAA0062, LZT-Hs4, NET34, Zrt-, Irt-like protein 14, Zinc transporter ZIP14, ZIP-14, ZIP14, Zrt- and Irt-like protein 14, Cig19

ID gène: 23516

Pathways: Transition Metal Ion Homeostasis